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不同消化液对PK-15细胞的影响

发表时间:2009-10-15  浏览量:1546  下载量:824
全部作者: 郑立勇,马凤龙,刘平平
作者单位: 山东信得科技有限公司;青岛农业大学生命科学学院
摘 要: 旨在寻找适合PK-15细胞的消化方法,实验比较了用0�25%,0�15%,0�10%,0�05%的胰蛋白酶和0�05%胰蛋白酶+0�53 mmol/L EDTA·4Na溶液消化PK-15细胞,观察PK-15细胞的增殖和传代效果,以确定不同消化液对PK-15细胞的消化效果。结果表明:0�05%胰蛋白酶+0�53 mmol/L EDTA·4Na溶液对PK-15细胞的消化效果最好,并且消化后细胞的生长情况较好。
关 键 词: 细胞生物学其他学科;PK-15细胞;细胞培养;胰蛋白酶;细胞消化
Title: Effect of different digestive solutions on PK-15 cells
Author: ZHENG Liyong, MA Fenglong, LIU Pingping
Organization: Shandong Sinder Technology Limited Company;College of Life Sciences, Qingdao Agriculture University
Abstract: This paper aims at searching for a suitable digestion method for PK-15 cells. PK-15 cells was digested by 0.25%, 0.15%, 0.10%, and 0.05% trypsin, and 0.05% trypsin+0.53 mmol/L EDTA�Na solution. The PK-15 cell proliferation and passage effects were observed, so as to determine the different digestion effects of the PK-15 cells in the digestive solutions. The results showed that the 0.05% trypsin+0.53 mmol/L EDTA�Na solution had the best digestion effect on the PK-15 cells, and the growth of the cells after the digestion is better.
Key words: cell biology in other disciplines; PK-15 cells; cell culture; trypsin; cell digestion
发表期数: 2009年10月第19期
引用格式: 郑立勇,马凤龙,刘平平. 不同消化液对PK-15细胞的影响[J]. 中国科技论文在线精品论文,2009,2(19):2072-2075.
 
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