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免疫扩散实验鉴定甲胎蛋白与热休克70蛋白的连接

发表时间:2009-12-15  浏览量:1501  下载量:694
全部作者: 丁美,赵军,张丽洁,段钟平,刘霜,陈煜
作者单位: 首都医科大学附属佑安医院北京市人工肝治疗培训中心;首都医科大学附属佑安医院病理中心
摘 要: 目的:应用琼脂免疫扩散实验鉴定甲胎蛋白(alphafetoprotein,AFP)与热休克70蛋白(heat shock protein 70,HSP70)是否交联。方法:在含0.1 mg/L溴酚蓝的凝胶琼脂板上,进行2次琼脂免疫扩散实验,第1次是用AFP-HSP70交联蛋白和AFP抗体进行琼脂免疫扩散,把扩散后沉淀带处理后再次和HSP70抗体进行琼脂免疫扩散。结果:2次均出现典型的沉淀带。结论:建立的方法简单、快捷,可适于临床和科研工作。
关 键 词: 免疫学;甲胎蛋白;热休克70蛋白;琼脂扩散实验
Title: Determination of the cross-linking between AFP and HSP 70 by agar gel double immunodiffusion test
Author: DING Mei, ZHAO Jun, ZHANG Lijie, DUAN Zhongping, LIU Shuang, CHEN Yu
Organization: Beijing Training and Treatment Center for Artificial Liver, Beijing Youan Hospital, Capital Medical University;Department of Pathology, Beijing Youan Hospital, Capital Medical University
Abstract: Objective: To identify the cross-linking of alphafetoprotein (AFP) and heat shock protein 70 (HSP70) via agar gel double immunodiffusion test (AGID). Methods: Agar gel immunodiffusion test was conducted twice in agar gel plate with 0.1 mg/L bromophenol blue, first, agar gel immunodiffusion test was carried out with AFP-HSP70 cross-linking protein and AFP antibody, then the test was conducted again with the processed precipitation band and HSP70 antibody. Results: The typical band appeared in the two agar gel immunodiffusion test. Conclusion: The method is simple, accurate, and is suitable for the application on clinical diagnosis and research.
Key words: immunology; alphafetoprotein; heat shock protein 70; agar gel diffusion test
发表期数: 2009年12月第23期
引用格式: 丁美,赵军,张丽洁,等. 免疫扩散实验鉴定甲胎蛋白与热休克70蛋白的连接[J]. 中国科技论文在线精品论文,2009,2(23):2457-2460.
 
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