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激光加工微通道内微流动溶液的荧光检测研究

发表时间:2010-02-15  浏览量:1406  下载量:449
全部作者: 白忠臣,秦水介,王德贵
作者单位: 贵州大学贵州省光电子技术及应用重点实验室
摘 要: 介绍了一种微米微通道中的静止溶液和微流动溶液的荧光检测新方法,研究微米级的微通道中溶液的微流动给荧光检测所带来的影响。实验结果表明:检测溶液在微米通道中单向流动,其接收到的荧光强度与静止溶液发出的荧光强度有所不同,两者比较而言,单向流动的溶液发出的荧光波动幅度大一些,且荧光强度整体增幅推后。基于此,该方法可以在微尺度下生物分析和生物检测中进行广泛研究与应用。
关 键 词: 光学;微通道;激光加工;荧光检测;石英晶体
Title: Study on fluorescent detection of microflowing solution using laser-processing quartz micro channels
Author: BAI Zhongchen, QIN Shuijie, WANG Degui
Organization: Guizhou Key Laboratory for Photoelectric Technology and Application, Guizhou University
Abstract: A novel method which is based on fluorescent detection of immovable and microflowing solution in micro channels is introduced. Moreover, the effect of microflowing solution in micro channels on fluorescent detection is researched in this paper. The experimental results show that receiving fluorescence intensity has been varied in microflowing solution comparing with immovable solution because of unidirectional flow of detecting solution in micro channels. At the same time, fluctuating ranges of receiving fluorescence intensity has been increased, and the whole amplification of receiving fluorescence intensity is laggard in time domain. The new technology based on the study can be applied extensively in bioanalysis and biological detection in micro-scale application.
Key words: optics; micro channel; laser-processing; fluorescent detection; quartz crystals
发表期数: 2010年2月第3期
引用格式: 白忠臣,秦水介,王德贵. 激光加工微通道内微流动溶液的荧光检测研究[J]. 中国科技论文在线精品论文,2010,3(3):215-218.
 
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