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改善酶固定以增强葡萄糖传感器的生物电化学活性

发表时间:2010-03-15  浏览量:1653  下载量:574
全部作者: 王依婷,俞磊,朱自强,娄蕾,杜伟杰,朱建中
作者单位: 华东师范大学科学与技术跨学科高等研究院;华东师范大学信息学院
摘 要: 研制了一种基于多壁碳纳米管(multi wall carbon nanotubes, MWCNTs)和ZnO纳米颗粒协同作用的高灵敏稳定的葡萄糖传感器。将ZnO颗粒淀积在带有负电荷的MWCNTs层上,由于等电点(isoelectric point, IEP)的差异,葡萄糖氧化酶(glucose oxidase, GOx)能够牢固地固定在ZnO 颗粒表面,最后将一层带正电的聚二烯丙基二甲基氯化铵(polydiallyldimethylammonium chloride, PDDA)覆盖在GOx上。这种特殊的自组装三明治结构(PDDA/GOx/ZnO/MWCNTs)能够很好地保持GOx的活性并防止酶泄漏。在0.05 mol/L的磷酸缓冲溶液(pH=6.8)中进行检测,电压为100 mV(vs. Ag/AgCl),用0.5 U GOx测得线性反应区域为0.1~16 mol/L,用2.0 U GOx测得灵敏度为50.2 mA·cm-2·mol-1·L-1,检测下限为250 nmol/L (3σ).通过葡萄糖传感器直接检测100例人血清与临床光学检测的结果比较发现,相关系数达到0.997.
关 键 词: 电化学;葡萄糖传感器;葡萄糖氧化酶;多壁碳纳米管;ZnO 纳米颗粒;协同作用
Title: Improved enzyme immobilization for enhanced bioelectrocatalytic activity of glucose sensor
Author: WANG Yiting, YU Lei, ZHU Ziqiang, LOU Lei, DU Weijie, ZHU Jianzhong
Organization: Advanced Research Centre of Interdisciplinary.Science and Technology, East China Normal University Development;Department of Information Science and Technology, East China Normal University
Abstract: A highly sensitive and stable glucose sensor based on the synergic action of multi wall carbon nanotube (MWCNTs) and ZnO nanoparticles has been developed. Glucose oxidase (GOx) is firmly immobilized on the ZnO nanoparticles surface due to their large difference in the isoelectric point (IEP), while the ZnO nanoparticles were deposited on the negatively charged MWCNTs layer. A cationic polydiallyldimethylammonium chloride (PDDA) layer was coated on the GOx layer. The unique sandwich-like layer structure (PDDA/GOx/ZnO/MWCNTs) formed by self-assembling provides a favorable microenvironment to keep the bioactivity of GOx and to prevent enzyme molecule leakage. Amperometric detection of glucose was carried out at 100 mV (vs. Ag/AgCl) in 0.05 mol/L phosphate buffer solution (pH=6.8) with a wide linear response range of 0.1 to 16 mmol/L by the sensor with 0.5 U GOx, with a sensitivity of 50.2 mA·cm-2·mol-1·L-1 and a detection limit of 250 nmol/L (3σ) by the sensor with 2.0 U GOx. Results obtained from the glucose sensor directly in human blood serum were compared with ones obtained by spectrometry by a local hospital. On testing 100 samples of serum, the correlation coefficient reaches 0.997.
Key words: electrochemistry;glucose sensor; glucose oxidase; MWCNTs; ZnO particles; synergic action
发表期数: 2010年3月第5期
引用格式: 王依婷,俞磊,朱自强,等. 改善酶固定以增强葡萄糖传感器的生物电化学活性[J]. 中国科技论文在线精品论文,2010,3(5):482-487.
 
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