您的位置:首页  > 论文页面

成体骨髓基质干细胞平面诱导为软骨样细胞的实验观察

发表时间:2008-02-15  浏览量:1769  下载量:546
全部作者: 杨欣,李健宁
作者单位: 北京大学第三医院;北京大学第三医院
摘 要: 本研究将兔骨髓基质干细胞分离培养,在体外进行软骨细胞定向平面诱导,应用细胞组织学、免疫组织化学检测。结果显示,在体外平面诱导条件下,成体兔骨髓基质干细胞可以定向分化为软骨样细胞,从细胞爬片组织染色及Ⅱ型胶原免疫组织化学检测可得以证实,提示在未来的研究中成体骨髓基质干细胞可平面诱导后作为软骨组织工程的种子细胞。
关 键 词: 组织工程;软骨;骨髓基质干细胞;种子细胞
Title: In vitro chondrogenesis of monolayer cultured bone mesenchymal stem cells
Author: YANG Xin,LI Jianning
Organization: Department of Plastic Surgery, Perking University Third Hospital
Abstract: The mesenchymal stem cells was isolated from adult rabbit’s bone marrow and monolayer cultured in vitro. Immunohistochemistry analyses and histological were performed, .The results showed that the induced cells expressed and produced collagen type II and cartilage associated matrix. This suggests that adult rabbit’s marrow-derived mesenchymal stem cells can differentiate into chondrocyte in monolayer culture. Bone marrow-derived mesenchymal stem cells are capable of chondrogenesis, as seed cell for future cartilage tissue engineering research is feasible.
Key words: tissue engineering, cartilage, bone mesenchymal stem cell,seed cell
发表期数: 2008年6月第3期
引用格式: 杨欣,李健宁. 成体骨髓基质干细胞平面诱导为软骨样细胞的实验观察[J]. 中国科技论文在线精品论文,2008,1(3):343-347.
 
2 评论数 0
暂无评论
友情链接