您的位置：首页 > 论文页面
高压氧、地塞米松对大鼠超长随意皮瓣FGF－1及TGFβ－1表达量的影响
发表时间：2008-02-15 浏览量：2232 下载量：617
| 全部作者： | 任纪祯，陈振雨，吴俐，苗Z年浚煸禄烤 |
| 作者单位： | 青岛大学医学院附属医院医学美容中心；青岛大学医学院附属医院医学美容中心；青岛大学医学院附属医院乳腺外科；青岛大学医学院附属医院高压氧科；青岛大学医学院附属医院高压氧科；青岛大学医学院附属医院高压氧科 |
| 摘 要： | 目的 探讨高压氧、常压氧对大鼠随意皮瓣成活率的影响。方法：Wister雄性大鼠60只，随机分为三组，每组于背部中线形成一蒂在尾端的超长随意皮瓣，A组不给于其他干预方式；B组术后即刻给予地塞米松5mg皮瓣下注射；C组术后当天开始每天给予高压氧2次，每次1 h，持续5天，第7天统一处理动物，计算皮瓣成活率。结果 术后7天测量各组大鼠平均成活率分别为：对照组：（48.6±8.0）%；地塞米松组成活率(81±4.4)%；高压氧组：(89.1±3.7)%；组间差异明显，p<0.05。高压氧组和地塞米松组较对照组VEGF及CD105表达明显提高，但高压氧组与地塞米松组之间没有差异性，而TGFβ-1则三组均为阴性或弱阳性，组间没有差异性。结论 高压氧与地塞米松均可以促进随意皮瓣的成活，但促进成活的机理可能存在差异。 |
| 关 键 词： | 临床医学；动物实验；随机分组；高压氧；常压氧；大鼠；随意皮瓣 FGF-1；TGFβ-1 |
| Title： | Research of the effect of hyperbaric oxygenation and normalbaric oxygenation on the survival rate of random-pattern flaps in rats |
| Author： | REN Jizheng, CHEN Zhenyu, WU Li, MIAO Yuanxin, ZHU Yuehua, FANG Jing |
| Organization： | The Affiliated Hospital of Medical College Qingdao University |
| Abstract： | Objective To study the effect of hyperbaric oxygenation and normalbaric oxygenation on the survival rate of random-pattern flaps in rats. [Methods] : Forty-five Wister rats were assigned to 3 groups equal in number randomly. A free flap was elevated in the central part of the rachis. Group A was left as controls. In group B, all the rats received normalbaric oxygenation twice a day until postoperative day 5. In group C, hyperbaric oxygenation was applied to the rats twice a day lasting for five days. On the seventh day, survival rates for all flaps were calculated. Results On the seventh day, the survival rates were as follows: control team,（48.6±8.0）%; normal baric oxygenation, (51.7±7.1)%; hyperbaric oxygenation team, (89.1±3.7)%.The difference was significant among the teams. Expression level of FGF-1 was much higher in the hyperbaric oxygenation team than the left two. And there is no significant difference among the three teams on the TGFβ-1 expression. Conclusion Hyperbaric oxygenation and dexamethasone used in this study significantly increase flap survival rate in rat random-pattern flap model, while the mechanism might be different. |
| Key words： | clinical medicine; animal studies; randomization; hyperbaric oxygenation; normalbaric oxygenation; rats; random flap; FGF-1; TGFβ-1 |
| 发表期数： | 2008年6月第3期 |
| 引用格式： | 任纪祯，陈振雨，吴俐，等. 高压氧、地塞米松对大鼠超长随意皮瓣FGF－1及TGFβ－1表达量的影响[J]. 中国科技论文在线精品论文，2008，1（3）：348-352. |
9
评论数 0请您登录
暂无评论
下载全文
多维论文