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赤芍水提物的药物血清对HSC-T6细胞促凋亡作用的实验研究

发表时间:2008-02-15  浏览量:1708  下载量:515
全部作者: 韩海啸,李军祥,杨晋翔,朱陵群,刘大新
作者单位: 北京中医药大学东方医院;北京中医药大学东方医院;北京中医药大学东直门医院;北京中医药大学东直门医院;北京大学第一临床医院
摘 要: 目的:应用流式细胞法,研究灌服赤芍水提物后的犬血清对乙醛造模后的肝星状细胞的促凋亡作用。方法:采用乙醛造模后的肝星状细胞株HSC-T6作为体外研究模型;以赤芍水提物给彼格犬1次性灌胃,取给药前的血清、给药后2 h、3 h血清作为实验药物血清。以流式细胞法检测以上采血时间点上2%浓度的药物血清对乙醛造模后的HSC-T6作用72 h后的促凋亡作用。结果:2 h、3 h两个时间点的含药血清能明显促进HSC-T6细胞的凋亡,和同样条件下用空白血清培养的HSC-T6细胞相比其凋亡比例(apoptosis, APOP)显著性增加(P<0.05)。在药物血清的作用下两个时点组的HSC-T6细胞在G0/G1期细胞比例明显上升(P<0.05);而S期细胞显著减少(P<0.05);但G2/M期并没有一致性的变化。结论:两个时点的药物血清对HSC-T6细胞都有显著的促凋亡作用,且能显著增加HSC-T6细胞在G0/G1期的比例及显著降低S期的比例,这可能是其抑制HSC-T6细胞增殖及促凋亡的原因之一。
关 键 词: 中药药理学;赤芍水提物;流式细胞术;肝星状细胞HSC-T6
Title: Apoptosis-promoting effect of Radix Paeoniae Rubra on HSC-T6 cells
Author: HAN Haixiao, LI Junxiang, YANG Jinxiang, ZHU Lingqun, LIU Daxin
Organization: Dongfang Hospital, Beijing University of Chinese Medicine;Dongzhimen Hospital, Beijing University of Chinese MedicineThe First Hospital of Beijing University
Abstract: Objective: To study the apoptosis-promoting effect of extract from Radix Paeoniae Rubra on hepatic stellate cell (HSC-T6)in the dog model. Methods: the hepatic stellate cell (HSC-T6) were taken as the model in vitro established by using acetaldehyde. The Radix Paeoniae Rubra extract was given to dog orally once only. The serum samples were taken before giving Radix Paeoniae Rubra extract, 2 and 3 hours after giving Radix Paeoniae Rubra extract respectvely. The apoptosis-promoting effect of these serum samples(in 2% concentration) on HSC-T6 after 72 hours in the detected by flow cytometry. Results: The serum sample promote obviously HSC-T6 apoptosis in 2 hour group and 3 hour group. The apoptosis rate was increased remarkably compare with that of the blank group (p<0.05). The proportion of HSC-T6 in G0/G1 phase were raised obviously in 2 hour group and 3 hour group(p<0.05), while reduced in S phase(p<0.05). There were no any changes in G2/M phase. Conclusion: The serum samples of Radix Paeoniae Rubra extract all have obvious apoptosis -promoting effects in the two time points, raise the proportion of HSC-T6 in G0/G1 phase and reduce in S phase, which may be the mechanism of Radix Paeoniae Rubra extract for inhibitingv HSC-T6 proliferation of cells and promoting its apoptosis.
Key words: Chinese pharmacology; Radix Paeoniae Rubra extract; flow cytometry; hepatic stellate cell (HSC-T6)
发表期数: 2008年6月第3期
引用格式: 韩海啸,李军祥,杨晋翔,等. 赤芍水提物的药物血清对HSC-T6细胞促凋亡作用的实验研究[J]. 中国科技论文在线精品论文,2008,1(3):353-357.
 
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