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HPLC法测定立血康软胶囊中大黄2组分的含量

发表时间:2008-02-15  浏览量:1513  下载量:680
全部作者: 陈大中
作者单位: 黑龙江中医药大学中医药研究院
摘 要: 目的:建立立血康软胶囊中大黄素和大黄酚的含量测定方法,以控制该产品的质量。方法:采用HPLC法,色谱柱:Kromasil C18柱(4.6 mm×250 mm,5 μm),柱温:30℃;流动相:甲醇 0.1%磷酸溶液(85:15),流速:1.0 mL/min,检测波长:254 nm。结果:大黄素在0.018 2~0.182 μg之间与其峰面积有良好的线性关系(r=0.999 9),大黄酚在0.044 2~0.442μg之间与其峰面积有良好的线性关系(r=0.999 9)。平均加样回收率为97.33%(RSD=1.44%)。结论:方法简便易行、准确可靠,可用于立血康软胶囊的质量控制。
关 键 词: 药剂学;大黄素;大黄酚;立血康软胶囊;含量测定
Title: Determination of contents of 2 components from Rhubarb in Lixuekang soft capsule by HPLC
Author: CHEN Dazhong
Organization: Research Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine
Abstract: Objective: To establish a quantitative determination method of Emodin and Chrysophanol in Lixuekang Soft Capsule to control quality. Methods: The method of HPLC was carried out with Kromasil C18 column (4.6 mm×250 mm, 5 μm), the mobile phase at a flow rate of 1.0 mL/min and detection wavelength was 254 nm. Results: Emodin and Chrysophanol had a good linearity in the range of 0.018 2~0.182 μg and 0.044 2~0.442 μg, respectively. Conclusion: The method is easy, accurate and can be used to control quality of Lixuekang Soft Capsule.
Key words: Pharmaceutics; Emodin; Chrysophanol; Lixuekang soft capsule; qualitative determination
发表期数: 2008年6月第3期
引用格式: 陈大中. HPLC法测定立血康软胶囊中大黄2组分的含量[J]. 中国科技论文在线精品论文,2008,1(3):363-366.
 
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