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黄芪多糖对慢性粒细胞白血病患者浆细胞样树突状细胞功能的影响

发表时间:2010-06-15  浏览量:1413  下载量:654
全部作者: 刘立民,柴晔,张连生
作者单位: 兰州大学第二医院血液·肿瘤科
摘 要: 目的:通过体外实验研究黄芪多糖(Astragalus polysaccharide,APS)对慢性粒细胞白血病(chronic myelocytic leukemia,CML)患者浆细胞样树突状细胞(plasmacytoid dendritic cells,pDC)功能的影响,为进一步阐明APS的免疫学活性及其在免疫治疗中的应用提供依据。方法:从慢性粒细胞白血病患者外周血分离单个核细胞,免疫磁珠法去除pDC,加入0,50,100,200 mg/L的APS共孵育24 h,收集上清液,应用酶联免疫吸附实验检测pDC分泌的IFN-α,IL-6,TNF-α量的变化。结果:实验应用慢性粒细胞白血病患者外周血来源的pDC,通过体外实验证明了APS能够提高治疗后的慢性期CML患者pDC分泌的IFN-α,IL-6,TNF-α量及初诊未治慢性期CML患者pDC分泌的IL-6,TNF-α量,并且呈APS浓度依赖性。结论:实验结果证实了APS能够增强慢性粒细胞白血病患者pDC的功能。
关 键 词: 血液病;黄芪多糖;慢性粒细胞白血病;浆细胞样树突状细胞;免疫治疗
Title: Regulation on function of plasmacytoid dendritic cells from chronic myelocytic leukemia by Astragalus polysaccharide
Author: LIU Limin, CHAI Ye, ZHANG Liansheng
Organization: Department of Hematology and Oncology, The Second Hospital of Lanzhou University
Abstract: Objective: To explore the mechanism of immunomodulatory activity of Astragalus polysaccharide (APS) on chronic myelocytic leukemia (CML) peripheral blood mononuclear cells (PBMC)-derived plasmacytoid dendritic cells (pDC) by in vitro experiment, and further explain immunomodulatory activity of APS and its application in immunotherapy. Methods: The experiment isolated chronic myelocytic leukemia peripheral blood mononuclear cell (PBMC) and isolated pDC by magnetic activated cell sorting (MACS), then incubated with APS (0, 50, 100, 200 mg/L) for 24 h, and used enzyme-linked immuno sorbent assay (ELISA) to determine the variation of IFN-α, IL-6, TNF-α excreted by pDC in supernate. Results: The experiment used pDC of peripheral blood of chronic myeloid leukemia (CML) patients, and proved by in vitro experiment that the APS could increase the concentration of IFN-α, IL-6, TNF-α secreted by pDC of the treated CML patients and the concentration of IL-6 and TNF-α secreted by pDC of the untreated CML patients, which depended on the APS concentration. Conclusion: APS can promote the function of pDC from CML.
Key words: hematonosis; Astragalus polysaccharide; chronic myelocytic leukemia; plasmacytoid dendritic cells; immunotherapy
发表期数: 2010年6月第11期
引用格式: 刘立民,柴晔,张连生. 黄芪多糖对慢性粒细胞白血病患者浆细胞样树突状细胞功能的影响[J]. 中国科技论文在线精品论文,2010,3(11):1113-1118.
 
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