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反相高效液相色谱分离16种多环芳烃

发表时间:2010-06-30  浏览量:22049  下载量:10914
全部作者: 徐恒振,褚亮亮,马新东,周传光,姚子伟
作者单位: 国家海洋环境监测中心
摘 要: 采用反相高效液相色谱-紫外检测器-荧光检测器(reversephase high performance liquid chromatography-ultraviolet detector-fluorescence detector,RP-HPLC-UVD-FLD)法,对16种多环芳烃(polycyclic aromatic hydrocarbons, PAHs)进行分离和检测,对流动相、流速、荧光吸收波长等条件进行优化。结果表明:除苯并(a)蒽和、二苯并[a,h]蒽和苯并[g,h,i]傒不能分开外,其他组分都能实现较好分离。该方法紫外检测保留时间的相对标准偏差(relative standard deviation, RSD)在0.48%~2.93%,响应值的RSD在0.59%~7.72%;荧光检测保留时间的RSD在0.09%~3.66%,响应值的RSD在0.39%~7.68%.PAHs的UVD最低检测限为0.019 3~0.773 9 ng;FLD最低检测限为0.001 5~2.017 9 ng.
关 键 词: 环境化学;多环芳烃;反相高效液相色谱;分离;检测
Title: Separation and determination of sixteen PAHs by reverse-phase high performance liquid chromatography
Author: XU Hengzhen, CHU Liangliang, MA Xindong, ZHOU Chuanguang, YAO Ziwei
Organization: National Marine Environmental Monitoring Center
Abstract: Reverse-phase high performance liquid chromatography-ultraviolet detector-fluorescent detector in series (RP-HPLC-UVD-FLD) was adopted to analyze and separate 16 polycyclic aromatic hydrocarbons (PAHs). The analysis conditions were optimized for mobile phase, the flow rate and fluorescent wavelength. The results showed that 16 PAHs were better separated off except benzo(a)anthracene and chrysene, dibenzo[a,b]anthracene and benzo[g,h,i]perylene. The relative standard deviation (RSD) of retain time and relative response factors of HPLC-UVD ranged from 0.48% to 2.93% and 0.59% to 7.72%, and the RSD of retain time and relative response factors of HPLC-FLD ranged from 0.09% to 3.66% and 0.39% to 7.68%, and the limits of detection of 16 PAHs of HPLC-UVD and HPLC-FLD ranged from 0.019 3 to 0.773 9 ng and 0.001 5 to 2.017 9 ng, respectively.
Key words: environmental chemistry; polycyclic aromatic hydrocarbons; reverse-phase high performance liquid chromatography; separation; determination
发表期数: 2010年6月第12期
引用格式: 徐恒振,褚亮亮,马新东,等. 反相高效液相色谱分离16种多环芳烃[J]. 中国科技论文在线精品论文,2010,3(12):1259-1265.
 
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