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血红蛋白在金-石墨烯修饰电极上的直接电化学

发表时间:2010-09-15  浏览量:1867  下载量:703
全部作者: 何亚萍,郑建斌,李矿田,盛庆林
作者单位: 西北大学分析科学研究所
摘 要: 构置了基于血红蛋白(hemoglobin, Hb)/离子液体(ionic liquid, IL)/金-石墨烯复合纳米材料(Gold-graphene nano composite material, Au-GE)/碳陶瓷(carbon ceramic electrode, CCE)修饰电极过氧化氢(H2O2)生物传感器,研究了Hb的直接电化学和电催化性质,建立了测定H2O2的伏安新方法。采用拉曼光谱法、紫外光谱法和电化学方法进行了表征。结果表明:血红蛋白在该修饰电极的循环伏安图有一对峰形较好、近似对称的氧化还原峰。H2O2在Hb/IL/Au-GE/CCE修饰电极上有明显的电催化现象,线性范围在2.7×10-7~ 2.5×10-4 mol/L,检测限为1.1×10-7 mol/L(S/N=3),米氏常数KappM为16.6 μmol/L.
关 键 词: 直接电化学;电催化;过氧化氢电化学传感器;血红蛋白;金-石墨烯复合纳米材料
Title: Direct electrochemistry of hemoglobin based on Au-GE modified carbon ceramic electrode
Author: HE Yaping, ZHENG Jianbin, LI Kuangtian, SHENG Qinglin
Organization: Institute of Analytical Science, Northwest University
Abstract: A new electrochemical biosensor based on the hemoglobin (Hb)/room temperature ionic liquid (IL)/Gold-graphene nano composite material(Au-GE)/carbon ceramic electrode (CCE) modified electrode was structured for the determination of hydrogen peroxide (H2O2). The direct electrochemistry and electrocatalytic of hemoglobin was studied and a new cyclic voltammetry method for the determination of H2O2 was formed. The resulting Au-GE composite film and the immobilization of Hb was characterized by Raman spectra, UV-vis, and electrochemical methods. Hb/IL/Au-GE/CCE showed a pair of well-defined, quasi-reversible redox peaks. It also showed good electrocatalytic ability to the reduction of H2O2 with the linear 2.7×10-7-2.5×10-4 mol/L and the detection limit was 1.1×10-7 mol/L (S/N=3). The apparent Michaelis-Menten constant (KappM) was estimated to be 16.6 μmol/L.
Key words: direct electrochemistry; electrocatalytic; biosensor of hydrogen peroxide; homoglobin; gold-graphene nano composite material
发表期数: 2010年9月第17期
引用格式: 何亚萍,郑建斌,李矿田,等. 血红蛋白在金-石墨烯修饰电极上的直接电化学[J]. 中国科技论文在线精品论文,2010,3(17):1742-1746.
 
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