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荧光淬灭法测定苏丹红I的方法研究

发表时间:2010-09-15  浏览量:1507  下载量:506
全部作者: 武天丽,王兴民
作者单位: 甘肃农业大学理学院
摘 要: 采用荧光分光光度计测定苏丹红I,结果表明:色氨酸与苏丹红I结合,从而使色氨酸的荧光淬灭,荧光淬灭程度在一定范围内与苏丹红I的质量浓度在0.001 4~0.009 5 mg/L(R=0.998 4)范围内呈线性关系,据此建立一种色氨酸荧光淬灭法快速测定辣椒及其制品中苏丹红I的含量。方法的检出限为0.000 7 mg/L. 用荧光淬灭法测定苏丹红I的方法新颖、快速、灵敏度高、选择性强、需样量少、简便,用于市售小尖椒和甘谷辣椒及其制品中苏丹红I的测定,结果满意。
关 键 词: 无机化学;苏丹红I;荧光淬灭;辣椒;乙腈;色氨酸
Title: Research on measuration of the sudan I by using fluorenscence quenching Processing method
Author: WU Tianli, WANG Xingmin
Organization: College of Sciences, Gansu Agricultural University
Abstract: After using fluorescence spectrophotometer to measure Sudan I, the reseults show that: when the tryptophan is combined with the Sudan I, the fluorenscence in the tryptophan will be quenched. Within a certain range of 0.001 4-0.009 5 mg/L(R=0.998 4), the fluorenscence quenching effects has a linear character of relationship with the thickness of Sudan I-acetonitrile, based on this situation a method of determining the Sudan I content quickly in the chili with the adoption of using the tryptophan fluorenscence quenching process has been established. The detection limit of this method is 0.000 7 mg/L, while the method of using the fluorenscence queching process to determine the Sudan I is original, fast, simple, with high delicacy and selectivity, and requires less resource. It is used to determine the Sudan I content in the Tine chili, the Gangu chili and their products, and the results are satisfying.
Key words: inorganic chemistry; Sudan I; fluorescence quenching; chili; acetonitrile; tryptophan
发表期数: 2010年9月第17期
引用格式: 武天丽,王兴民. 荧光淬灭法测定苏丹红I的方法研究[J]. 中国科技论文在线精品论文,2010,3(17):1757-1762.
 
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