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东方百合和亚洲百合若干品种离体快繁和再生体系建立的研究

发表时间:2010-09-30  浏览量:1414  下载量:655
全部作者: 李南,吴沙沙,刁义维,吕英民,张启翔
作者单位: 北京林业大学园林学院,国家花卉工程技术研究中心
摘 要: 以东方百合(Oriental hybrids)的‘西伯利亚’(‘Siberia’)、‘索邦’(‘Sorbonne’)和亚洲百合(Asiatic hybrids)的‘精粹’(‘Elite’)为实验材料,进行百合的离体快繁和再生体系建立的研究。快繁研究获得适合 ‘西伯利亚’、‘索邦’和‘精粹’的最佳诱导培养基、继代培养基和根诱导培养基。此外,磷用量为MS基本培养基的1.5倍时有利于鳞茎的膨大及根系生长。添加1.5 mg/L的多效唑(PP333)有利于鳞茎增长。再生体系建立研究得出:‘西伯利亚’、‘索邦’适宜的愈伤组织诱导培养基为MS+6-BA 0.5 mg/L+NAA 1.0 mg/L和MS+6-BA 1.0 mg/L+NAA 0.5 mg/L,愈伤组织适宜的再生培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L. ‘精粹’适宜的愈伤组织诱导培养基为MS+6-BA 1.0 mg/L+2,4-D 1.0 mg/L+NAA 0.1 mg/L,再生培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.
关 键 词: 观赏园艺;百合;离体快繁;再生体系
Title: Study on the rapid micropropagation and the establishment of regeneration system of several Oriental hybrids lily and Asiatic hybrids lily cultivars
Author: LI Nan, WU Shasha, DIAO Yiwei, LV Yingmin, ZHANG Qixiang
Organization: College of Landscape Architecture, Beijing Forestry University, National Engineering Research Center for Floriculture
Abstract: Rapid micropropagation and regeneration system of Oriental hybrids lily and Asiatic hybrids lily cultivars were studied. Oriental hybrids lily cultivars ‘Siberia’, ‘Sorbonne’ and Asiatic hybrids lily cultivar ‘Elite’ were selected as the material. These studies were divided into two parts, one is rapid micropropagation, and the other is the establishment of regeneration system. The results show that: The rapid micropropagation research has obtained the optimum growth culture of explants, proliferation culture and rooting culture for ‘Siberia’, ‘Sorbonne’ and ‘Elite’. The bulblets were enlarged and root growth was promoted by increasing phosphorus concentration to 1.5 times of standard MS. The bulblets were also enlarged by using 1.5 mg/L PP333. The following were resulted from the research of the establishment of regeneration system. The optimum inducement media for callus from scales of ‘Siberia’ and ’Sorbonne’ were as follows: MS+6-BA 0.5 mg/L+NAA 1.0 mg/L and MS+6-BA 1.0 mg/L+NAA 0.5 mg/L, the optimum medium for the regeneration of callus was as follows: MS+6-BA 0.5 mg/L+NAA 0.1 mg/L. The fitting inducement medium for callus from scales of ‘Elite’ was: MS+6-BA 1.0 mg/L+2,4-D 1.0 mg/L+NAA 0.1 mg/L, the optimum medium for the regeneration of callus was: MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.
Key words: ornamental horticulture; lily; rapid micropropagation; regeneration system
发表期数: 2010年9月第18期
引用格式: 李南,吴沙沙,刁义维,等. 东方百合和亚洲百合若干品种离体快繁和再生体系建立的研究[J]. 中国科技论文在线精品论文,2010,3(18):1893-1903.
 
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