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N-Boc-Glu的合成方法优化

发表时间:2010-10-15  浏览量:2028  下载量:720
全部作者: 杨明峰,蒋珍菊,赵萍,赵旭壮,吴灿军
作者单位: 兰州理工大学生命科学与工程学院;西华大学生物工程学院
摘 要: 以甲醇作为均相反应介质,Boc酸酐与谷氨酸为原料,在碱性条件下,以产物熔点、羧基含量及产物纯度作为产物质量确定的指标,最终得出N-Boc-Glu的最优合成条件。通过实验小样确定反应时间为23 h左右;反应物料的物质的量配比(Glu∶Boc2O)为1.0∶1.5;反应温度为30℃;pH值为8~9;结晶条件是向溶液中加入乙酸乙酯(EtOAc)后再酸化的最优合成条件。
关 键 词: 生物化学;Boc酸酐;谷氨酸;合成;方法优化
Title: Optimization of synthesis method of N-Boc-Glu
Author: YANG Mingfeng, JIANG Zhenju, ZHAO Ping, ZHAO Xuzhuang, WU Canjun
Organization: School of Life Science and Engineering, Lanzhou University of Technology; School of Bioengineering, Xihua University
Abstract: The paper takes methanol as homogeneous reaction medium, Boc-anhydride and glutamic acid as material, and alkaling condition as reaction condition. The optimal synthesis condition was determined by measuring the melting point, the content of carboxylgroup and the product purity. The results show that the best experiment condition is as follows: The proportion of adding amount proportion of reactants (Glu∶Boc2O) is 1.0∶1.5, the temperature of the reaction is room temperature, the time of the reaction is about 23 hours, the pH value is 8-9, and the crystallization condition is the optimal synthesis condition of acidification after EtOAc is added to the solution.
Key words: biochemistry; Boc-anhydride; glutamic acid; synthesis; optimization of method
发表期数: 2010年10月第19期
引用格式: 杨明峰,蒋珍菊,赵萍,等. N-Boc-Glu的合成方法优化[J]. 中国科技论文在线精品论文,2010,3(19):1978-1982.
 
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