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清肝抑纤饮防治大鼠肝纤维化的实验研究

发表时间:2010-12-15  浏览量:1273  下载量:601
全部作者: 贾爱芹,尹常健
作者单位: 济南军区总医院消化科;山东省中医院肝病科
摘 要: 目的:研究清肝抑纤饮防治大鼠肝纤维化的疗效,并探讨其作用机制。方法:将110只大鼠随机分为9组。除空白对照组外均给予皮下注射40% CCl4-花生油溶液,同时给予相应中药灌胃。8周后处死大鼠,检测各组血清肝功能、肝纤维化指标:肝组织匀浆丙二醛(malondialdehyde, MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)活性,TNF-α,TGF-β1,MMP-2及TIMP-2含量;RT-PCR法检测肝组织TGF-β1 mRNA表达;末端标记法(terminal-deoxynucleoitidyl transferase mediated nick end labeling,TUNEL)检测肝星状细胞凋亡;免疫组化法观察α-SMA在各组大鼠肝组织内的表达。结果:1) 清肝抑纤饮可显著改善肝脏炎症程度及减轻脂质过氧化损伤,清热解毒及凉血活血药物起主要作用;2) 可使肝组织中α-SMA表达明显降低,清热解毒与凉血活血药物的配伍起主要作用;3) 可从mRNA到蛋白水平降低TGF-β1表达;4) 可降低肝组织TNF-α含量,清热解毒药物起主要作用;5) 可使TIMP-2降低,凉血活血药物起主要作用。结论:清肝抑纤饮可通过改善肝脏炎症、抑制肝星状细胞活化、降低促肝纤维化因子表达等方面防治肝纤维化,其中清热解毒与凉血活血药物的配伍起主要作用。
关 键 词: 中西医结合医学;清肝抑纤饮;肝纤维化;防治;实验研究
Title: Experimental study on curative effect of Qingganyixian decoction on hepatic fibrosis
Author: JIA Aiqin, YIN Changjian
Organization: Gastroenterology Department, General Hospital of Jinan Military Area; Hepatology Department, Shandong Province Hospital of Traditional Chinese Medicine
Abstract: Objective: To observe the preventive and therapeutic effects on rat hepatic fibrosis of Qinganyixian decoction, and discuss its mechanism of action. Methods: Divide 110 Wistar rats into 9 groups. The control group was given normal diet, free drinking water. Give the remaining groups subcutaneous injection with 40% CCl4-oil solution twice a week, and lavage them the herbs or water for 8 weeks. Detect the liver function and hepatic fibrosis indexes. Detect liver tissue homogenate malondialdehyde (MDA) content and superoxide dismutase (SOD) activity, assay the content of TNF-α, TGF-β1, MMP-2 and TIMP-2, detect the expression of TGF-β1 mRNA using RT-PCR, and test the apoptosis of HSC with terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) step detection. Results: 1) Qinganyixian decoction can significantly decrease the liver inflammation and lipid peroxidation, where Qingrejiedu herbs and Liangxuehuoxue herbs play the most important role. 2) Qinganyixian decoction can decrease the expression of α-SMA positive cells significantly, where Qingrejiedu herbs and Liangxuehuoxue herbs play the most important role. 3) Qinganyixian decoction can reduce the expression of TGF-β1 from mRNA to protein level significantly. 4) Qinganyixian decoction can significantly reduce the TNF-α level, especially the Qingrejiedu herbs. 5) Qinganyixian decoction can reduce TIMP-2, where Liangxuehuoxue herbs play the major role. Conclusion: Qingganyixian decoction can prevente and treate hepatic fibrosis by improving liver inflammation, inhibiting the activation of HSC and promote apoptosis of activated HSC, and reducing the expression of hepatic fibrosis promoting factors, wherein Qingrejiedu herbs and Liangxuehuoxue herbs play the most important role.
Key words: medicine combined with Chinese traditional medicine and western medicine; Qingganyixian decoction; liver fibrosis; prevention and treatment; experimental study
发表期数: 2010年12月第23期
引用格式: 贾爱芹,尹常健. 清肝抑纤饮防治大鼠肝纤维化的实验研究[J]. 中国科技论文在线精品论文,2010,3(23):2455-2460.
 
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