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铜离子促进PrP形成细胞毒性聚集

发表时间:2011-03-15  浏览量:1503  下载量:807
全部作者: 吴頔,张文韬,罗侃,王文晞,黄力勤,陈锐,黄涛,肖庚富
作者单位: 武汉大学生命科学学院现代病毒学研究中心,病毒学国家重点实验室;中国科学院武汉病毒研究所,病毒学国家重点实验室
摘 要: 研究证明生理酸性条件下铜离子在细胞型朊蛋白(cellular prion protein, PrP)的聚集过程中起到关键作用。经过孵育后,PrP形成聚集沉淀,圆二色光谱(circular dichroism, CD)以及外源荧光结果显示铜离子促进了PrP二级结构的转变。静态光散射结果证明PrP形成聚集。四甲基偶氮唑盐微量酶反应比色法(MTT)实验显示,该条件下孵育得到的PrP聚集产物对神经母细胞瘤细胞SK-N-SH具有毒性。以上结果表明:铜离子能够促进PrP形成具有神经细胞毒性的聚集,这一研究为揭示PrP疾病过程中铜离子所起到的作用提供了一些有益的线索。
关 键 词: 微生物学;朊病毒;铜离子;生理酸性环境;聚集;细胞毒性
Title: Copper ions promote neurotoxic aggregation of PrP
Author: WU Di, ZHANG Wentao, LUO Kan, WANG Wenxi, HUANG Liqin, CHEN Rui, HUANG Tao, XIAO Gengfu
Organization: State Key Laboratory of Virology, Modern Virology Research Centre, College of Life Sciences, Wuhan University; State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
Abstract: It was indicated that Cu2+ was a vital factor involved in the aggregation of cellular prion protein (PrP). Cu2+ triggered structural conversion revealed by circular dichroism (CD) spectra and fluorescence measurement. Scattering results confirmed the aggregation of PrP. MTT assay demonstrated that PrP aggretates induced significant damage of SK-N-SH cells. It was indicated that at physiological acidic environment Cu2+ promoted the aggregation of PrP, which were cytotoxic to neuron cells. And these researches may offer some clues for Cu2+ in prion diseases.
Key words: microbiology; prion; copper ion; physiological acidic environment; aggregation; cytotoxicity
发表期数: 2011年3月第5期
引用格式: 吴頔,张文韬,罗侃,等. 铜离子促进PrP形成细胞毒性聚集[J]. 中国科技论文在线精品论文,2011,4(5):435-440.
 
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