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利用定点诱变法初步确定玉米CMS-S不育基因的毒性区域

发表时间:2011-03-15  浏览量:1259  下载量:1025
全部作者: 汪峰,于江华,赵衍鑫,肖海林
作者单位: 华中农业大学生命科学技术学院;华中农业大学作物遗传改良国家重点实验室
摘 要: 通过易错PCR介导的随机突变和重叠延伸PCR介导的定点突变技术,获得了5条orf355-orf77突变序列。将这些序列转化大肠杆菌,检测外源片段的表达对大肠杆菌的毒性。发现其中3条突变序列编码的多肽对大肠杆菌无毒性,这3个突变位点均位于orf355读码框内,说明orf355所表达的多肽对大肠杆菌的生长具有毒性作用,而orf77内不存在这样的毒性区域。
关 键 词: 分子生物学;CMS-S;定点诱变;毒性区域
Title: Preliminary determination of toxic region of sterile gene in maize CMS-S by site-directed mutagenesis
Author: WANG Feng, YU Jianghua, ZHAO Yanxin, XIAO Hailin
Organization: College of Life Science and Technology, Huazhong Agricultural University; National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University
Abstract: Five mutated sequences of orf355-orf77 were obtained via random mutation mediated by error-prone PCR and site-directed mutation mediated by overlap extension PCR. The mutated orf355-orf77 sequences were transformed into E.coli and their toxicity was detected. Three mutated sequences could encode peptides without toxicity to E.coli. The mutated nucleotides of these non-toxic sequences all located in the orf355 region, indicating that the peptide encoded by orf355 was toxic to E.coli, while the orf77 didn’t show this kind of toxicity.
Key words: molecular biology; CMS-S; point mutation; toxic region
发表期数: 2011年3月第5期
引用格式: 汪峰,于江华,赵衍鑫,等. 利用定点诱变法初步确定玉米CMS-S不育基因的毒性区域[J]. 中国科技论文在线精品论文,2011,4(5):453-458.
 
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