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利用定点诱变法初步确定玉米CMS-S不育基因的毒性区域
发表时间：2011-03-15 浏览量：1699 下载量：1183
| 全部作者： | 汪峰，于江华，赵衍鑫，肖海林 |
| 作者单位： | 华中农业大学生命科学技术学院；华中农业大学作物遗传改良国家重点实验室 |
| 摘 要： | 通过易错PCR介导的随机突变和重叠延伸PCR介导的定点突变技术，获得了5条orf355-orf77突变序列。将这些序列转化大肠杆菌，检测外源片段的表达对大肠杆菌的毒性。发现其中3条突变序列编码的多肽对大肠杆菌无毒性，这3个突变位点均位于orf355读码框内，说明orf355所表达的多肽对大肠杆菌的生长具有毒性作用，而orf77内不存在这样的毒性区域。 |
| 关 键 词： | 分子生物学；CMS-S；定点诱变；毒性区域 |
| Title： | Preliminary determination of toxic region of sterile gene in maize CMS-S by site-directed mutagenesis |
| Author： | WANG Feng, YU Jianghua, ZHAO Yanxin, XIAO Hailin |
| Organization： | College of Life Science and Technology, Huazhong Agricultural University; National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University |
| Abstract： | Five mutated sequences of orf355-orf77 were obtained via random mutation mediated by error-prone PCR and site-directed mutation mediated by overlap extension PCR. The mutated orf355-orf77 sequences were transformed into E.coli and their toxicity was detected. Three mutated sequences could encode peptides without toxicity to E.coli. The mutated nucleotides of these non-toxic sequences all located in the orf355 region, indicating that the peptide encoded by orf355 was toxic to E.coli, while the orf77 didn’t show this kind of toxicity. |
| Key words： | molecular biology; CMS-S; point mutation; toxic region |
| 发表期数： | 2011年3月第5期 |
| 引用格式： | 汪峰，于江华，赵衍鑫，等. 利用定点诱变法初步确定玉米CMS-S不育基因的毒性区域[J]. 中国科技论文在线精品论文，2011，4（5）：453-458. |
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