您的位置:首页  > 论文页面

低温下棉纤维蔗糖合成酶表达特征及其与蔗糖/纤维素含量的关系

发表时间:2011-03-15  浏览量:1094  下载量:557
全部作者: 王友华,郑密,陈兵林,束红梅,周治国
作者单位: 南京农业大学农学院,农业部南方作物生理生态重点开放实验室
摘 要: 以科棉1号为材料,通过双向电泳、质谱分析等技术研究低温条件下棉纤维中蔗糖合成酶蛋白含量、酶活性、基因表达强度的变化及其与蔗糖和纤维素含量变化的关系。结果显示:蔗糖合成酶含量及其酶活性在10 DPA(day postanthesis)时较低,15 d时达到最大值,20 d时有所下降;其基因的表达强度在10,15 d时均较高,20 d时开始下降;低温促进酶蛋白含量的增加及酶基因的表达。10,15,20 d时蔗糖含量逐渐下降,纤维素含量快速上升,低温使蔗糖含量下降速度和纤维素含量增加速度变缓。上述结果表明:尽管低温对蔗糖合成酶有显著的诱导作用,但低温下其催化能力下降,导致蔗糖含量上升,纤维素含量下降。蔗糖合成酶对低温的敏感性是纤维发育对低温敏感的关键性原因。
关 键 词: 作物栽培学与耕作学;棉花;蔗糖合成酶;蔗糖;纤维素
Title: Changes of sucrose synthase and its relation with the content of sucrose and cellulose in cotton fiber under low temperature condition
Author: WANG Youhua, ZHENG Mi, CHEN Binglin, SHU Hongmei, ZHOU Zhiguo
Organization: Key Laboratory of Crop Physiology & Ecology of Southern China, Ministry of Agriculture, Nanjing Agricultural University
Abstract: With cotton cultivar KM-1 as material, specific enzyme activity, protein content and gene expression manner of sucrose synthase in the fiber cells were examined, and their relationship with sucrose and cellulose content were analyzed by dimensional electrophoresis and mass spectrum analysis. Data showed that, both the protein content and the specific enzyme activity were the lowest on 10 d, and the highest on 15 d, while the gene expression density was higher on both 10, 15 d and lower on 20 d. Low temperature environment could induce the gene expression and protein accumulation. Sucrose content decreased from 10 d to 20 d, while cellulose content increased in this stage. Low temperature could alleviate the changing rate for both sucrose and cellulose content. The results indicated that, low temperature could induce the expression of sucrose synthase coding gene, while it could reduce the transformation of sucrose to cellulose, which was catalyzed by sucrose synthase, and then resulted in the enhancement of sucrose content and the decline of cellulose content. The sensitivity of fiber development to low temperature was majored due to the sensitivity of sucrose synthase to low temperature.
Key words: Crop cultivation and farming system; Gossypium hirsutum L.; sucrose synthase; sucrose; cellulose
发表期数: 2011年3月第5期
引用格式: 王友华,郑密,陈兵林,等. 低温下棉纤维蔗糖合成酶表达特征及其与蔗糖/纤维素含量的关系[J]. 中国科技论文在线精品论文,2011,4(5):475-482.
 
0 评论数 0
暂无评论
友情链接