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通脉大生片对肾虚排卵障碍不孕模型大鼠卵巢基质金属蛋白酶-9的影响

发表时间:2011-06-15  浏览量:1290  下载量:613
全部作者: 马洁,傅春华,黄金珠,方云芸
作者单位: 成都中医药大学临床医学院;成都中医药大学科技处;合肥市肥东县人民医院中医科
摘 要: 目的:探讨通脉大生片治疗前后对雄激素致不孕大鼠(androgen-sterilized rats,ASR)模型卵巢基质金属蛋白酶-9(matrix metalloproteinases 9,MMP-9)表达的变化。方法:采用ASR模型,以原位杂交方法观察分析卵巢MMP-9 mRNA信号表达的情况。结果:由于药物的干预,各个用药组MMP-9阳性细胞的表达浓度和表达总量均高于模型组,以通脉大生片高剂量组卵巢MMP-9 mRNA表达尤其明显,与正常组水平接近,差异无统计学意义(P>0.05)。通脉大生片高剂量组卵巢MMP-9 mRNA表达亦明显优于氯米芬组,差异有统计学意义(P<0.05)。结论:通脉大生片可能通过促进肾虚排卵障碍不孕大鼠卵巢基质金属蛋白酶-9的表达,发挥其对不孕症的治疗作用,这可能是其促进卵泡发育和卵巢排卵的机制之一。
关 键 词: 妇产科学;通脉大生片;卵巢基质金属蛋白酶-9;排卵障碍;不孕症;雄激素致不孕大鼠模型
Title: Effects of Tongmai Dasheng tablet on MMP-9 about rat renal deficiency induced ovulatory infertility
Author: MA Jie, FU Chunhua, HUANG Jinzhu, FANG Yunyun
Organization: Clinical Medical School, Chengdu University of Traditional Chinese Medicine; Science and Technology Agency, Chengdu University of Traditional Chinese Medicine; Traditional Chinese Medicine Department, Hefei Feidong People’s Hospital
Abstract: Objective: To explore the matrix metalloproteinases 9 (MMP-9)’s change of androgen-sterilized rats (ASR) before and after Tongmai Dasheng tablet treatment. Methods: ASR was induced, and MMP-9 expression was detected with situ hybridization. Results: Because of drug intervention, the concentration and total amount of expression in each drug group were much greater than that in model group. The expression of MMP-9 in the high dosage group of Tongmai Dasheng tablet was as high as that in the control group (P>0.05). The expression of MMP-9 in the high dosage group of Tongmai Dasheng tablet was much higher than that in clomiphene citrate group (P<0.05). Conclusion: To rat renal deficiency induced ovulatory infertility, Tongmai Dasheng tablet can promote the expression of MMP-9 and play a role in the treatment of infertility. This may be one of the mechanisms on promoting ovarian follicle development and ovulation.
Key words: gynecotokology; Tongmai Dasheng tablet; matrix metalloproteinase-9; ovulation disorder; infertility; androgen-sterilized rats model
发表期数: 2011年6月第11期
引用格式: 马洁,傅春华,黄金珠,等. 通脉大生片对肾虚排卵障碍不孕模型大鼠卵巢基质金属蛋白酶-9的影响[J]. 中国科技论文在线精品论文,2011,4(11):997-1001.
 
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