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TRPV6在蛋鸡不同肠段和肾脏中的定位表达

发表时间:2011-06-15  浏览量:1331  下载量:604
全部作者: 杨俊花,周振雷,邓益峰,侯加法
作者单位: 南京农业大学动物医学院;上海市农业科学院农产品质量标准与检测技术研究所
摘 要: 目的:探明瞬时性受体电位香草精受体6(transient receptor potential vanilloid receptor 6,TRPV6)蛋白在蛋鸡肠道和肾脏中的组织学分布特点。方法:采用石蜡切片、特异性的TRPV6抗体对高产蛋鸡不同肠段和肾脏组织进行免疫组织化学方法研究,同时采用Western blotting方法研究TRPV6蛋白在这些部位的浓度变化。结果:在十二指肠、空肠、回肠、盲肠和直肠的刷状缘绒毛上皮均有TRPV6阳性表达分布,直肠阳性表达较少,肠隐窝或杯状细胞则偶有表达。在肾脏中,TRPV6在近曲小管、远曲小管和集合小管均有阳性表达。免疫印迹(Western blotting)结果显示,十二指肠TRPV6含量最高,直肠含量最低,其他肠段位于二者之间。肾脏TRPV6含量也低于十二指肠。结论:TRPV6蛋白在蛋鸡整个肠道和肾上皮具有阳性表达,表明Ca2+跨细胞转运在蛋鸡体内肠道Ca2+吸收或肾脏重吸收都具有重要作用。
关 键 词: 兽医学;蛋鸡;瞬时性受体电位香草精受体6;肠道;肾脏;定位
Title: The localization expression of TRPV6 in different intestinal segments and kidney in laying hens
Author: YANG Junhua, ZHOU Zhenlei, DENG Yifeng, HOU Jiafa
Organization: College of Veterinary Medicine, Nanjing Agricultural University; Institute for Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences
Abstract: Objective: the study was aimed to investigate the distribution of transient receptor potential vanilloid receptor 6 (TRPV6) in intestine and kidney of laying hens. Methods: immunohistochemical analysis was used to investigate the TRPV6 localization and expression in different intestinal segments and kidney in laying hens during peak laying. In addition, changes of TRPV6 concentration among these tissues were obtained by the Western blotting analysis. Results: TRPV6 was localized to the brush-border membranes of the duodenum, jejunum, ileum, cecum and rectum. Expression was weaker in the rectum and little or no expression was found in crypt and goblet cells. In the kidney, distinct immunopositive staining for TRPV6 was detected at the apical domain of the proximal convoluted tubules (PCT), distal convoluted tubules (DCT) and medullary connecting tubules (CNT). Furthermore, Western blotting analysis indicated that the greatest amount of TRPV6 was expressed in the duodenum and the least in the rectum, expressing intermediate levels were in the other segments. Moreover, the kidney presented lower TRPV6 protein levels compared to duodenum. Conclusion: the epithelial Ca2+ channel TRPV6 is strongly expressed in the apical cells of the entire intestine and the renal tubules, suggesting that active Ca2+ transcellular transport plays a crucial role in dietary calcium (re)absorption in laying hens.
Key words: veterinary medicine; laying hens; transient receptor potential vanilloid receptor 6; intestine; kidney; localization
发表期数: 2011年6月第11期
引用格式: 杨俊花,周振雷,邓益峰,等. TRPV6在蛋鸡不同肠段和肾脏中的定位表达[J]. 中国科技论文在线精品论文,2011,4(11):1025-1032.
 
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