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山东泰安茶区土壤硝化细菌的筛选及分离鉴定

发表时间:2011-10-15  浏览量:1466  下载量:646
全部作者: 于亚伟,黄晓琴,王乃栋,韩晓阳,张丽霞
作者单位: 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室
摘 要: 通过菌株的富集培养和分离纯化,从山东茶园土壤中筛选出3株亚硝酸菌(A1~A3)和3株硝酸菌(N1~N3)。经测定氨氮去除率和亚硝酸盐氮去除率,得出各菌株的硝化活性依次为:A3>A1>A2,N2>N1>N3.将活性较强的A3和N2进行生理生化特征的测定,初步鉴定A3为亚硝化单胞菌属,N2为硝化刺菌属。此外,研究了温度、pH值对菌株A3和N2硝化活性的影响。结果表明:2种菌的最适温度均在30℃;而最适pH值不同,其中A3为8.0,N2为7.5.
关 键 词: 茶学;茶园土壤;鉴定;硝化细菌
Title: Screening and identification of nitrobacteria from tea garden soil in Taian, Shandong
Author: YU Yawei, HUANG Xiaoqin, WANG Naidong, HAN Xiaoyang, ZHANG Lixia
Organization: State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University
Abstract: By the experimental procedure of enrichment culture and microbial purification, three nitrosobacteria (A1-A3) and three nitrobacteria (N1-N3) were screened from tea garden soil in Shandong province. According to the removal rate of ammonia nitrogen and nitrite nitrogen, the nitrifying activity of strains arranged as fallows: A3>A1>A2, N3>N1>N2. On the basis of the characteristics of strain’s physiological and biochemistry, the strain A3 was preliminary identified as Nitrosomonas and N2 as Nitrospina. In addition, the effect of temperature and pH on the nitrifying activity of A3 and N2 were measured. The result showed that the optimum temperature of two strains were 30℃; but their optimal pH were different, A3 for 8.0, N2 for 7.5.
Key words: tea science; tea garden soil; identification; nitrobacteria
发表期数: 2011年10月第19期
引用格式: 于亚伟,黄晓琴,王乃栋,等. 山东泰安茶区土壤硝化细菌的筛选及分离鉴定[J]. 中国科技论文在线精品论文,2011,4(19):1749-1754.
 
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