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秦岭细粘束孢内切葡聚糖苷酶基因LQ-CX克隆和序列分析

发表时间:2011-10-15  浏览量:1194  下载量:356
全部作者: 陈辉,唐明,高瑞霞
作者单位: 西北农林科技大学林学院
摘 要: 通过聚合酶链式反应(polymerase chain reaction,PCR)扩增得到秦岭细粘束孢(Leptographium qinlingensis Tang et Chen)内切葡聚糖苷酶基因LQ-CX序列。序列分析表明:所克隆的LQ-CX片段长742 bp,BLAST发现LQ-CX的核苷酸序列与西洋梨(Pyrus communis)的内切-1,4-β-D-葡聚糖苷酶AB084463.1序列和纤维素酶AB067644.1序列同源性较高,均为80%,目的片段的氨基酸序列与桉树(Eucalyptus globules)AAZ08322.1和杨树(Populus tremuloides)AAS45400.1的内切-1,4-β-葡聚糖苷酶序列同源性均达85%,说明秦岭细粘束孢的内切葡聚糖苷酶基因可能与其寄主树木华山松表现为协同进化。通过DNAMAN 5.2.2软件对LQ-CX基因和NCBI数据库中来源于其他真菌的内切葡聚糖苷酶基因序列进行比对,得到的LQ-CX的核苷酸序列与青霉(Penicillium chrysogenum)和白曲霉(Aspergillus kawachii)的进化距离比较近,推测秦岭细粘束孢纤维素酶基因的进化可能与真菌的种属进化相关,其CX酶基因与青霉、白曲霉的纤维素酶基因可能属于同一类型。
关 键 词: 森林保护学;秦岭细粘束孢;纤维素酶;基因克隆;华山松大小蠹
Title: Cloning and sequence analysis of endo-β-1,4-glucanase gene of Leptographium qinlingensis
Author: CHEN Hui, TANG Ming, GAO Ruixia
Organization: College of Forestry, Northwest A & F University
Abstract: The experiment uses polymerase chain reaction (PCR) for amplifying the 742 bp endo-β-1,4-glucanases gene fragment LQ-CX of Leptographium qinlingensis Tang et Chen. Sequence analysis showed that the cloned LQ-CX segment had length of 742 bp. BLAST of the nucleotide sequence showed that the nucleotide sequence homology with the published Pyrus communis endo-1,4-β-glucosidase gene (GenBank No. AB084463.1 and AB067644.1) was 80%, amino acid sequence homology shared 85% with Eucalyptus globules (AAZ08322.1) and Populus tremuloides (AAS45400.1). It showed that the cellulose gene of L.qinlingensis might be co-evoluted with the host pine trees. Homology analysis of sequence from LQ-CX and other 6 cellulase of GenBank available with DNAMAN5.2.2 showed that LQ-CX gene was closely related to Penicillium chrysogenum and Aspergillus kawachii. It shows that the evolution of cellulose gene of L.qinlingensis may be related to the evolution of species, and the CX gene and P. chrysogenum, A. kawachii cellulase gene may belong to the same type.
Key words: forest conservation; Leptographium qinlingensis; cellulase; gene clone; Dendroctonus armandi
发表期数: 2011年10月第19期
引用格式: 陈辉,唐明,高瑞霞. 秦岭细粘束孢内切葡聚糖苷酶基因LQ-CX克隆和序列分析[J]. 中国科技论文在线精品论文,2011,4(19):1775-1780.
 
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