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海洋植物大叶藻EST序列的获得与分析

发表时间:2011-11-15  浏览量:1895  下载量:670
全部作者: 孔凡娜,刘利民,茅云翔
作者单位: 中国海洋大学海洋生命学院
摘 要: 为了解大叶藻(Zostera marina)的耐盐机制,发掘其耐盐基因,构建大叶藻的全长cDNA文库,通过5′末端测序获得了4 002条高质量的表达序列标签(expressed sequence tags,EST),被组装成241个片段重叠群(contigs)和1 673个克隆子(singletons),代表了1 914个非重复序列(unigenes)。BLASX程序分析显示1 664个unigenes与NCBI nr数据库中的已知基因具有较高的相似性,其中丰度最高的基因为金属硫蛋白(metallothionein)和叶绿素a/b结合蛋白(chlorophyll a/b-binding protein),分别占总EST的7.1%和1.7%. 蛋白相邻类聚簇(clusters of orthologous groups of proteins, COG)分析显示499个基因被注释,KEGG(Kyoto encyclopedia of genes and genomes)分析表明,1 462个基因被分配到161个生化途径中,938个基因得到GO(gene ontology)注释,InterProScan程序分析产生1 003个InterPro家族。相关结果有助于进一步从分子水平理解大叶藻的耐盐机制。
关 键 词: 水产生物学;大叶藻;全长cDNA文库;表达序列标签;耐盐基因
Title: Generation and analysis of expressed sequence tags from the salt-tolerant eelgrass species Zostera marina
Author: KONG Fanna, LIU Limin, MAO Yunxiang
Organization: College of Marine Life Sciences, Ocean University of China
Abstract: To investigate salt-tolerant mechanism and discover salt-tolerant genes of Zostera marina, a cDNA library was constructed in which single-pass 5′ sequencing of 4 081 clones yielded 4 002 high-quality expressed sequence tags (ESTs). These ESTs were assembled into 241 contigs and 1 673 singletons, representing 1 914 unigenes. Average length of these ESTs was 582 bp, ranging from 100 bp to 1 500 bp. BLASX analysis revealed that 1 664 unigenes had significant homology to known genes against the NCBI nr database (E value≤10-5). Among them, the two most abundant genes encoded metallothionein (157 ESTs) and chlorophyll a/b-binding protein (38 ESTs), which accounted for 7.1% and 1.7% of total ESTs, respectively. Compared to the Clusters of orthologous groups (COG) categories, 499 unigenes were annotated (E value≤10-10). Using the Kyoto encyclopedia of genes and genomes (KEGG), 1 462 of the 1914 unigenes were assigned into 161 pathways (E value≤10-5). For gene ontology (GO) hierarchy analysis, a total of 938 unigenes were assigned GO terms. For InterProScan searches, 1 003 InterPro families were recognized. Results of this study will contribute to further understanding of the molecular mechanism of saline tolerance in Z. marina.
Key words: aquaculture biology; Zostera marina; cDNA library; expressed sequence tags; salt-tolerant gene
发表期数: 2011年11月第21期
引用格式: 孔凡娜,刘利民,茅云翔. 海洋植物大叶藻EST序列的获得与分析[J]. 中国科技论文在线精品论文,2011,4(21):2013-2021.
 
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