您的位置：首页 > 论文页面
AluYb8MUTYH变异体导致细胞DNA 8-OHdG水平升高——中国人群2型糖尿病遗传风险研究
发表时间：2011-12-15 浏览量：1721 下载量：432
| 全部作者： | 陈慧梅，孙彩霞，郭文文，孟然，杜宏，齐秋锋，顾昕，李丽荣，张奎，朱大龙，王亚平 |
| 作者单位： | 南京大学医学院，江苏省医学分子技术重点实验室；南京大学医学院金陵医院；南京大学医学院附属鼓楼医院 |
| 摘 要： | 研究MUTYH基因的一个常见变异（AluYb8MUTYH）在2型糖尿病（type 2 diabetes mellitus，T2DM）发病中的作用。对565例2型糖尿病患者和565例健康对照进行病例-对照分析，发现AluYb8MUTYH在糖尿病患者和对照中的分布不同，糖尿病患者中突变等位基因（P）的百分率明显增高［44.7%对40.3%，P=0.003，优势比(odds ratio, OR)=1.199］，但在早发性或迟发性糖尿病患者之间没有差别。检测了66例2型糖尿病患者白细胞DNA中8-羟基脱氧鸟苷（8-OHdG）的含量，结果表明：每百万个dG中MUTYH基因野生型、AluYb8MUTYH杂合型和突变纯合型个体的8-OHdG含量分别为10.4，15.9和22.3，突变型与野生型相比P<0.001.因此，认为AluYb8MUTYH多态性可以作为2型糖尿病一个新的遗传风险因素，同时8-OHdG的积累也促进了2型糖尿病的发生。 |
| 关 键 词： | 医学遗传学；MUTYH基因；8-羟基脱氧鸟苷；2型糖尿病；基因多态性 |
| Title： | AluYb8MUTYH variant increases 8-OHdG level in cell DNA: research on genetic risk of type 2 diabetes in Chinese |
| Author： | CHEN Huimei, SUN Caixia, GUO Wenwen, MENG Ran, DU Hong, QI Qiufeng, GU Xin LI Lirong, ZHANG Kui, ZHU Dalong, WANG Yaping |
| Organization： | Jiangsu Key Laboratory of Molecular Medicine, Medical School, Nanjing University; Jinling Hospital, Medical School of Nanjing University; Medical School Affiliated Drum Tower Hospital, Nanjing University |
| Abstract： | This paper investigated the common variant of the MUTYH gene (AluYb8MUTYH) for its possible role in type 2 diabetes mellitus (T2DM). A total of 565 T2DM patients and 565 healthy subjects were enrolled in a case-control study. Statistic analysis showed that the distribution of AluYb8MUTYH differed in diabetic patients from controls, with a moderately increased percentage of the mutant allele (P) ［44.7% versus 40.3%, P=0.033, odds ratio (OR)=1.199］. However, this distribution was similar between the diabetic early-onset and late-onset subgroups. In addition, 66 T2DM patients were further evaluated for 8-hydroxy-2’deoxyguanosine (8-OHdG) levels in genomic DNA. The average value of 8-OHdG in one million of deoxyguanosines was 10.4 in patients with the wild-type genotype, 15.9 with heterozygote, and 22.3 with homozygote variation respectively (P<0.001, compared with the wild-type). Therefore, the AluYb8MUTYH variant was a novel genetic risk factor for T2DM, and accumulated 8-OHdG could contribute to this disease. |
| Key words： | medical genetics; MUTYH gene; 8-OHdG; type 2 diabetes mellitus; gene polymorphism |
| 发表期数： | 2011年12月第23期 |
| 引用格式： | 陈慧梅，孙彩霞，郭文文，等. AluYb8MUTYH变异体导致细胞DNA 8-OHdG水平升高——中国人群2型糖尿病遗传风险研究[J]. 中国科技论文在线精品论文，2011，4（23）：2019-2025. |
0
评论数 0请您登录
暂无评论
下载全文
多维论文