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不同产地杉叶总黄酮含量的测定

发表时间:2011-12-15  浏览量:1054  下载量:299
全部作者: 刘进鹏,范灵婧,吴纯洁
作者单位: 成都中医药大学药学院;上海交通大学农业与生物学院
摘 要: 目的:建立杉叶中总黄酮含量的测定方法。方法:采用分光光度法,以穗花杉双黄酮为标品,NaNO2-Al(NO3)3-NaOH法显色,对杉叶中总黄酮进行含量测定,检测波长为400 nm. 结果:总黄酮的回归方程为y=0.059 3x+0.014 7,r=0.999 7,线性范围2.24~13.44 mg/L. 结论:方法操作简便,结果准确、灵敏、重现性好,可用于杉叶中总黄酮的定量分析,并为其开发提供依据。
关 键 词: 中药化学;杉叶;总黄酮;穗花杉双黄酮;分光光度法
Title: Determination of total flavonoid in leaves of Cunninghamia lanceolata from different regions
Author: LIU Jinpeng, FAN Lingjing, WU Chunjie
Organization: College of Pharmacy, Chengdu University of Traditional Chinese Medicine; School of Agriculture and Biology, Shanghai Jiao Tong University
Abstract: Objective: To establish a method for the determination of total flavonoids in leaves of Cunninghamia lanceolata. Methods: Amentoflavone was the reference substance, and chromogenic with NaNO2-Al(NO3)3-NaOH method, the experiment was performed by spectrophotometric method with a wavelength at 400 nm. Results: The standard curves of amentoflavone was y=0.059 3x+0.014 7 (r=0.999 7). The linear detection concentration range of total flavonoids was 2.24~13.44 mg/L. Conclusion: The method is simple, accurate and suitable for the determination of total flavonoids in C. lanceolata. The results provide a basis for the development and application of C. lanceolata.
Key words: chemistry of Chinese materia medica; leaves of Cunninghamia lanceolata; total flavonoid; amentoflavone; spectrometry
发表期数: 2011年12月第23期
引用格式: 刘进鹏,范灵婧,吴纯洁. 不同产地杉叶总黄酮含量的测定[J]. 中国科技论文在线精品论文,2011,4(23):2172-2175.
 
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