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补肾调肝法对ROS模型小鼠INHb-ACT-FS系统的影响
发表时间：2011-12-15 浏览量：1386 下载量：780
| 全部作者： | 黄文玲，金哲，李玲，李云波 |
| 作者单位： | 北京中医药大学东方医院；北京中医药大学第三附属医院 |
| 摘 要： | 目的：探讨补肾调肝方乌萸汤对卵巢不敏感综合征（resistant ovarian syndrome，ROS）小鼠卵巢抑制素B（inhibin-B，INHb）-激活素（activin，ACT）-卵泡抑素（follistatin，FS）系统的影响。方法：以小鼠卵透明带3（zona pellucida 3, ZP3）建立ROS模型。用乌萸汤药低、中、高剂量进行治疗，以戊酸雌二醇组、模型组为阳性对照，双抗体夹心酶联免疫吸附技术（Sandwitch ELISA）检测各组小鼠血清INHb，ACT，FS的表达。结果：模型对照组血清INHb，FS含量降低，ACT升高，与空白对照组比较有显著性差异（P<0.05）；用药各组INHb，FS含量均升高，ACT降低，较模型组有显著性差异（P＜0.05）；乌萸汤高剂量组INHb，FS含量高于西药组，其差异无统计学意义（P＞0.05）；乌萸汤各剂量组ACT含量均高于西药对照组，其差异无统计学意义（P＞0.05）。结论：乌萸汤可改善模型小鼠免疫性卵巢炎症状并改善卵巢功能，说明补肾调肝中药可以调节卵巢内部因子，从而改善卵巢生殖内分泌功能。 |
| 关 键 词： | 中医妇科学；卵巢不敏感综合征；补肾调肝；动物实验；抑制素B-激活素-卵泡抑素 |
| Title： | Effects of tonifying kidney and regulating liver on expression of INHb-ACT-FS protein in blood serum of immune ROS mice |
| Author： | HUANG Wenling, JIN Zhe, LI Ling, LI Yunbo |
| Organization： | Dongfang Hospital of Beijing University of Chinese Medicine; Third Affiliated Hospital of Beijing University of Chinese Medicine |
| Abstract： | Objective: To probe the effects of Wuyu Decoction (WYT), a Chinese compound recipe for tonifying kidney and regulating liver, on expression of inhibin-B (INHb)-activin (ACT)-follistatin (FS) protein in mice blood serum with immune resistant ovarian syndrome (ROS). Methods: BALB/c female mice model of ROS was created by multiple sites subcutaneous injection of zona pellucida 3 (ZP3) to mice, and treated with low, middle and high dosage WYT, to detect the expression of INHb-ACT-FS protein in blood serum by ELISA method. Results: Comparing with the normal group, the expression of INHb, FS decreased and the expression of ACT increased obviously in model group, statistically significant (P＜0.05). Comparing with the model group, the expression of INHb, FS increased and the expression of ACT decreased obviously in medication group, statistically significant (P＜0.05). The expression of INHb, FS increased in the mice of dosage WYT than in the estradiol valerate group statistical insignificant (P＞0.05). Comparing with the group of estradiol valerate, the expression of ACT increased in blood serum in the mice of WYT, statistically insignificant (P＞0.05). Conclusion: WYT can relieve ovarian immune inflammation and improve ovarian function. It proves that tonifying kidney and regulating liver can adjust internal factors of ovarian, thus controlling reproduction and endocrine function. |
| Key words： | gynecology of Chinese medicine; resistant ovarian syndrome; tonifying kidney and regulating liver; animal experiment; inhibin-B-activin-follistatin |
| 发表期数： | 2011年12月第23期 |
| 引用格式： | 黄文玲，金哲，李玲，等. 补肾调肝法对ROS模型小鼠INHb-ACT-FS系统的影响[J]. 中国科技论文在线精品论文，2011，4（23）：2176-2179. |
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