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藏药三果汤DPPH体外抗氧化作用及HPLC指纹图谱初步研究

发表时间:2011-12-15  浏览量:1056  下载量:503
全部作者: 姚喆,张艺,杨继家,罗尚华,兰莎,唐策
作者单位: 成都中医药大学民族医药学院
摘 要: 目的:采用体外1,1-二苯基-2-三硝基苯肼(DPPH)对藏药三果汤的抗氧化能力进行测定,并结合HPLC指纹图谱,实现对三果汤抗氧化作用的初步评价。方法:利用紫外分光光度法与DPPH抗氧化评价体系对不同来源的三果汤药材、单味药对照药材及其化学成分没食子酸、没食子酸乙酯、鞣花酸进行评价,并建立了三果汤HPLC指纹图谱分析方法。结果:不同来源的三果汤抗氧化活性差异不大,其所含化学成分以没食子酸抗氧化能力最强;通过对不同来源的三果汤药材进行HPLC指纹图谱分析,发现没食子酸含量明显高于其他2种成分。提示没食子酸可能是三果汤抗氧化的主要活性成分之一。结论:结合HPLC指纹图谱,采用DPPH紫外分光光度法对藏药三果汤及其抗氧化成分活性进行初步研究,具有简便、有效的特点,为进一步探索三果汤药效物质基础提供了科学依据。
关 键 词: 民族医学;藏药;三果汤;1,1-二苯基-2-三硝基苯肼;抗氧化;指纹图谱
Title: Preliminary study on DPPH antioxidant capability in vitro and the HPLC fingerprints of Tibetan medicine Sanguo decoction
Author: YAO Zhe, ZHANG Yi, YANG Jijia, LUO Shanghua, LAN Sha, TANG Ce
Organization: College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine
Abstract: Objective: Adopting DPPH in vitro to determine the oxidation resistance of Tibetan medicine Sanguo decoction and evaluating the overall antioxidation with the HPLC fingerprints. Methods: Medicinal materials from different origins in Sanguo decoction, single reference material as well as the chemical ingredients gallic acid, ellagic acid and ethyl gallate in them were evaluated by ultraviolet (UV) spectrophotometry and DPPH oxidation-system. The analytic technique for HPLC fingerprints was established as well. Results: Sanguo decoction from different resources had no obvious difference in antioxidation activity, and antioxidant ability of gallic acid IC50 was the strongest among its chemical components. Based on the HPLC fingerprint analysis, it is indicated that the gallic acid content was higher than those of the other two components, and it was concluded that gallic acid might be one of the main active constituents of Sanguo decoction. Conclusion: This paper combined exoteric-DPPH with HPLC fingerprints to analyze the antioxidant contents of Sanguo decoction. The method is convenient and effective to provide a scientific basis for finding out the active ingredients of Sanguo decoction.
Key words: national medicine; Tibetan medicine; Sanguo decoction; DPPH; antioxidation; fingerprint
发表期数: 2011年12月第23期
引用格式: 姚喆,张艺,杨继家,等. 藏药三果汤DPPH体外抗氧化作用及HPLC指纹图谱初步研究[J]. 中国科技论文在线精品论文,2011,4(23):2185-2191.
 
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