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河流水陆交错带土壤中厌氧氨氧化菌的原位鉴别

发表时间:2011-12-31  浏览量:1683  下载量:687
全部作者: 王咨元,裴元生
作者单位: 北京师范大学环境学院
摘 要: 采用16S rDNA方法,对河北省府河黄花沟及安州位点水陆交错带的土壤样品,进行厌氧氨氧化(anatrobic ammonium oxidation, ANAMMOX)菌的原位鉴别,初步建立样品的16S rDNA克隆文库,共包括6个16S rDNA克隆序列。利用ClustalX软件对克隆序列进行了序列分析及系统发育树的绘制。分析结果表明:府河水陆交界面土壤样品中含有与已知ANAMMOX菌Candidatus “Jettenia asiatica”相似性为93%的16S rDNA基因,经鉴定为厌氧氨氧化菌Brocadia分支的新种类。样品中还含有组成Planctomycetes新分支的16S rRNA基因,它们与已知ANAMMOX菌序列的遗传距离较远,其微生物特性还有待进一步研究。该研究也为ANAMMOX过程及ANAMMOX菌在淡水水体氮转化新途径的研究提供了参考。
关 键 词: 环境工程;氮循环;厌氧氨氧化菌;河流水陆交错带;分子生物学技术
Title: In-situ identification of anaerobic ammonium oxidation bacteria in freshwater sediments
Author: WANG Ziyuan, PEI Yuansheng
Organization: School of Environment, Beijing Normal University
Abstract: In-situ identification of ANAMMOX bacteria was conducted using 16S rDNA approach for sediment samples from the Fu River in Hebei Province, China. 16S rDNA clone library including 6 clone sequences were constructed. The alignment of these sequences and treeing were conducted using ClustalX. Results show that the sediment samples contained 16S rDNA genes closely related to the known ANAMMOX bacterium Candidatus “Jettenia asiatica”(similarity of 93%). They also contained 16S rRNA gene sequences from a new branch of Planctomycetes distantly related to the ANAMMOX sequence cluster. However, the microbiological characteristics of these Planctomycetes are to be studied in the future. The detection of ANAMMOX bacteria will lead to further research on ANAMMOX process in the remediation and restoration of freshwater aquatic ecosystems and new understanding of the nitrogen cycle.
Key words: environmental engineering; nitrogen cycle; anaerobic ammonium oxidation bacteria; freshwater sediment; molecular techniques
发表期数: 2011年12月第24期
引用格式: 王咨元,裴元生. 河流水陆交错带土壤中厌氧氨氧化菌的原位鉴别[J]. 中国科技论文在线精品论文,2011,4(24):2203-2208.
 
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