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突变体在研究细菌VBNC状态发生机制中的作用与优势

发表时间:2012-03-15  浏览量:1159  下载量:541
全部作者: 李影,王伟利,孟庆峰
作者单位: 吉林农业大学动物科学技术学院;吉林出入境检验检疫局检验检疫技术中心
摘 要: 处于活的非可培养(viable but non-culturable,VBNC)状态维持期内的细菌生物学性质难以稳定存在,且极易在研究过程中消失,转入真正死亡期。因而,有关VBNC发生机制的研究也只停留在某一(些)已知基因在正常与VBNC下表达情况的比较,尚未能作出客观评述。为此,综述细菌VBNC作用机制的研究进展,分析突变体在研究VBNC发生机制的优点及作用,提出利用变性高效液相色谱(denaturing high performance liquid chromatography,DHPLC)与扩增片段长度多态性(amplified fragment length polymorphism,AFLP)技术筛选突变体,并论证2项技术的可行性,旨在为研究者提供进一步研究的思路与方法。�
关 键 词: 微生物学;突变体;综述;细菌活的非可培养状态;分子发生机制�
Title: Roles and advantages of mutant in molecular mechanism of VBNC bacteria
Author: LI Ying, WANG Weili, MENG Qingfeng
Organization: College of Animal Science, Jilin Agriculture University; Inspection and Quarantine Technology Center, Jilin Entry-Exit Inspection and Quarantine Bureau
Abstract: It is difficult to research mechanism of viable but non-culturable (VBNC) bacteria, because VBNC bacteria are unstable, and they easily get death and disappearance. Some expressing known genes were analyzed by comparing normal and VBNC state, but VBNC mechanism wasn’t still objectively expounded. Mechanism progress, mutant advantages and mutant detection technology with denaturing high performance liquid chromatography (DHPLC) and amplified fragment length polymorphism (AFLP) are reviewed in order to provide new method and thought for researchers.�
Key words: microbiology; mutant; reviews; viable but non-culturable state; molecular mechanism
发表期数: 2012年3月第5期
引用格式: 李影,王伟利,孟庆峰. 突变体在研究细菌VBNC状态发生机制中的作用与优势[J]. 中国科技论文在线精品论文,2012,5(5):387-391.
 
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