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杭州湾湿地滩涂的微生物多样性研究

发表时间:2012-03-15  浏览量:1205  下载量:598
全部作者: 张静微,徐旭,杨季芳
作者单位: 浙江理工大学生物工程研究所;南京工业大学生物与制药工程学院;浙江万里学院生物与环境学院
摘 要: 为探究杭州湾湿地微生物的群落结构和呰草(Scirpus triqueter)对微生物多样性的影响,采用PCR-DGGE技术对2011年1月从浙江杭州湾慈溪湿地采集的9个样品进行微生物多样性的分析研究。结果表明:1) 9个样品回收条带的测序结果表明,样品的优势菌种主要归属于6个门类,分别为变形菌门(Proteobacteria)(δ-变形菌纲,γ-变形菌纲,β-变形菌纲)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、蓝藻门(Cyanobacteria)、厚壁菌门(Firmicutes)和酸杆菌门(Acidobacteria),特别是变形菌群(Proteobacteria),占总数的36.2%;2) 变性梯度凝胶电泳(denaturing gradient gel electrophoresis, DGGE)电图谱揭示出呰草植被覆盖下的土壤样品微生物群落结构明显比裸滩处样品丰富;3) 中潮位和高潮位微生物群落组成类似,不同深度,组成不同,均表现为表层和深层的微生物组成有一定程度的差异。
关 键 词: 微生物学;微生物多样性;群落结构;PCR-变性梯度凝胶电泳;湿地�
Title: Analysis on wetlands microbial diversity of the beach in Hangzhou Bay
Author: ZHANG Jingwei, XU Xu, YANG Jifang
Organization: Institute of Bioengineering, Zhejiang Sci-Tech University; School of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology; College of Biological and Environmental Sciences, Zhejing Wanli University
Abstract: In order to describe the structure of bacterial communities in Hangzhou Bay and explore the impact of Scirpus triqueter on microbial diversity, nine samples were collected from Cixi wetlands in Zhejiang in January 2011, and microbial community structure was evaluated by PCR-DGGE method. The results showed that: 1) The microbial community was mainly consisted of Proteobacteria (Delta proteobacteria, Gamma proteobacteria, Beta proteobacteria), Bacteroidetes, Actinobacteria, Cyanobacteria, Firmicutes and Acidobacteria in Hangzhou Bay wetlands, and Proteobacteria accounted for 36.2%; 2) DGGE denaturing gradient gel electrophoresis fingerprinting revealed that the microbial community structures of samples from the soil covered by S. triqueter were significantly richer than that from the bare beach; 3) The medium tide and high tide had similar structures of microbial communities in the S5 station, and the main difference was the compositions of microbial communities of surface layer and deep layer.�
Key words: microbiology; microbial diversity; community structure; PCR-DGGE; wetlands
发表期数: 2012年3月第5期
引用格式: 张静微,徐旭,杨季芳. 杭州湾湿地滩涂的微生物多样性研究[J]. 中国科技论文在线精品论文,2012,5(5):392-400.
 
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