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血浆/血清中游离核酸的检测方法及临床应用进展

发表时间:2012-03-15  浏览量:1625  下载量:791
全部作者: 潘旻,葛芹玉
作者单位: 东南大学生物电子学国家重点实验室;东南大学儿童发展与学习科学教育部重点实验室
摘 要: 血浆/血清中游离核酸的发现为相关的临床应用研究提供了新的思路,特别是在肿瘤和无创性产前诊断等领域中更是具有广泛的应用前景,因此备受研究者和临床医生的关注。而高效的分离纯化则是更好地利用的前提,许多研究者提出了游离核酸高效分离纯化的方法,并且有些已经开发成商品化试剂盒,这里根据该研究进展对游离核酸的分离纯化及检测方法做简单综述,并且就其在临床上的最新应用进展进行概述,为进一步更好地将游离核酸应用于临床和科研工作中奠定基础。
关 键 词: 分子生物学;游离核酸;综述;实时荧光定量PCR;检测方法;临床应用
Title: Progress in detection method and clinical application of plasma/serum circulating nucleic acid
Author: PAN Min, GE Qinyu
Organization: State Key Laboratory of Bioelectronics, Southeast University; Key laboratory of Child Development and Learning Science, Ministry of Education, Southeast University
Abstract: Cell free nucleic acid namely circulating nucleic acid was more and more concerned since it was discovered. Many approaches for the separation and detection of circulating nucleic acid were improved, and some of them were commercialized. In this paper, by reviewing the recent studies, the separation and detection method of circulating nucleic acid in plasma and serum were summarized, and its clinical application was also outlined which provided foundation for further application and researches.
Key words: molecular biology; circulating nucleic acid; reviews; real-time quantitative PCR; detection method; clinical application
发表期数: 2012年3月第5期
引用格式: 潘旻,葛芹玉. 血浆/血清中游离核酸的检测方法及临床应用进展[J]. 中国科技论文在线精品论文,2012,5(5):401-407.
 
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