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AhAREB1蛋白转录活性初步研究
发表时间:2012-03-15 浏览量:1777 下载量:537
全部作者: | 姚瑶,刘旭,洪岚,李天丰,朱明鲲,李玲 |
作者单位: | 华南师范大学生命科学学院,广东省植物发育生物工程重点实验室 |
摘 要: | 设计了一系列不同的AhAREB1基因缺失片段载体转入酵母中,利用酵母单杂交方法,通过观察菌株生长和半乳糖苷酶显色,确定AhAREB1转录因子结构域的功能。结果表明:AhAREB1转录因子C端无转录活性,N端保守结构域是转录激活区域,其非保守区域和C4保守区域具有抑制自身转录激活的作用。� |
关 键 词: | 分子生物学;转录活性;酵母单杂交;AhAREB1;转录因子;花生� |
Title: | Preliminary study on transcriptional activity of AhAREB1 protein |
Author: | YAO Yao, LIU Xu, HONG Lan, LI Tianfeng, ZHU Mingkun, LI Ling |
Organization: | Guangdong Provincial Key Lab of Biotechnoogy for Plant Development, College of Life Sciences, South China Normal University |
Abstract: | This study created several vectors of truncated AhAREB1 gene fragment to transform into yeast, using the yeast one-hybrid method of yeast growth and X-β-Gal experiment to confirm the domain function of AhAREB1 transcription factor. These results indicate that the C terminal of AhAREB1 transcription factor has not transcriptional activity but the N terminal has, while the non-conserved and C4 conserved domain can inhibit its own transcriptional activation.� |
Key words: | molecular biology; transcriptional activation; yeast one-hybrid; AhAREB1; transcription factor; peanut |
发表期数: | 2012年3月第5期 |
引用格式: | 姚瑶,刘旭,洪岚,等. AhAREB1蛋白转录活性初步研究[J]. 中国科技论文在线精品论文,2012,5(5):408-414. |

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