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基于叶绿体DNA trnL-trnF序列研究云南省绞股蓝属植物的亲缘关系

发表时间:2012-03-15  浏览量:1394  下载量:460
全部作者: 董文华,刘占林,黄兆辉,杨佳,苏海伦,李忠虎,赵桂仿
作者单位: 西部资源生物与现代生物技术教育部重点实验室,西北大学生命科学学院
摘 要: 通过叶绿体DNA trnL-trnF测序分析,研究云南省绞股蓝属(Gynostemma BL.)7种(14个居群)植物的遗传变异。结果显示:绞股蓝属植物叶绿体DNA trnL-trnF序列变异长度为732~810 bp,变异主要由2个位置片段的插入或缺失引起,共鉴定得到5种单倍型。绞股蓝属植物不同居群的样品在碱基含量、信息位点的碱基位置和遗传距离等方面存在一定差异;具有相同地理分布的不同物种拥有相同的单倍型,说明其祖先物种可能存在多态性。分子变异分析表明大部分遗传变异主要存在于居群间。系统发育分析表明绞股蓝属植物的亲缘关系与地理分布具有一定的相关性,但也存在不一致现象。推测生态因素以及绞股蓝不同物种复杂的倍性变化可能是导致特定遗传结构形成的重要原因。因而,要确定不同绞股蓝物种之间的合理亲缘关系还需要结合其他方面的证据支持。�
关 键 词: 植物学;绞股蓝属植物;DNA测序;遗传变异;物种分布;亲缘关系�
Title: Phylogenetic relationships of Gynostemma BL. in Yunnan based on chloroplast DNA trnL-trnF sequence variation
Author: DONG Wenhua, LIU Zhanlin, HUANG Zhaohui, YANG Jia, SU Hailun, LI Zhonghu, ZHAO Guifang
Organization: Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University
Abstract: 7 species (14 populations) of Gynostemma BL. collected in Yunnan province were analyzed by the chloroplast DNA (cpDNA) trnL-trnF sequencing technologies. The length of cpDNA trnL-trnF sequence was varied to 732-810 bp. Insertion and/or deletion at two positions mainly caused the genetic variation among populations of Gynostemma BL., 5 cpDNA haplotypes were uncovered within samples of Gynostemma BL. Some differences of the cpDNA sequence were detected in the base contents, positions of the parsimony informative sites and genetic distance among populations of Gynostemma BL. from samples collected from different areas in Yunnan. The different Gynostemma BL. species distributed in the same areas shared the same cpDNA haplotypes. It concluded that the ancestral species of Gynostemma BL. may be polymorphic. Analysis of molecular variance indicated that the most of genetic variations were existed among populations. Phylogenetic analysis showed that some correlations were found between genetic relationships and geographic locations among different Gynostemma BL. species, whereas the discrepancies were also detected among them. It concluded that the ecological factors and the complex changes in cytotypes of Gynostemma BL. species might have caused the formation of specific genetic structure among populations. So other evidence is needed to be combined with for ascertaining the reasonable genetic relationships among Gynostemma BL. species.
Key words: botany; Gynostemma BL.; DNA sequencing; genetic variation; species distribution; phylogenetic relationship
发表期数: 2012年3月第5期
引用格式: 董文华,刘占林,黄兆辉,等. 基于叶绿体DNA trnL-trnF序列研究云南省绞股蓝属植物的亲缘关系[J]. 中国科技论文在线精品论文,2012,5(5):415-422.
 
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