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日本文昌鱼(Branchiostoma japonicum) AmphiH2A-core和AmphiH2A.X基因的克隆及其表达分析

发表时间:2012-03-15  浏览量:1046  下载量:427
全部作者: 李光,王秀,刘晓慧,王义权
作者单位: 厦门大学生命科学学院
摘 要: 在前期构建了日本文昌鱼(Branchiostoma japonicum)性腺差显文库,并获得了一系列精巢、卵巢特异表达的候选基因的基础上,对其中一个卵巢特异表达基因AmphiH2A.X及其同源基因AmphiH2A-core的进化和表达进行系统分析,发现两者在基因结构和编码蛋白的一级序列方面都与其对应的脊椎动物同源基因高度相似,如AmphiH2A.X含有哺乳动物H2A.X的典型标志性结构——SQEY(Ser-Gln-Glu-Tyr)功能域;与脊椎动物相同,日本文昌鱼AmphiH2A-core也属于广谱型表达基因,但与脊椎动物不同的是AmphiH2A.X特异表达于日本文昌鱼的卵巢中,且其表达量随着卵巢成熟度的提高而不断增长。由此可以推断:H2A-core在日本文昌鱼和脊椎动物中有相似的功能,而H2A.X在日本文昌鱼中的主要功能可能是参与其卵巢的发育。�
关 键 词: 遗传学;日本文昌鱼;AmphiH2A-core; AmphiH2A.X;卵巢发育
Title: Sequence identification and expression pattern of AmphiH2A-core and AmphiH2A.X genes in amphioxus (Branchiostoma japonicum)
Author: LI Guang, WANG Xiu, LIU Xiaohui, WANG Yiquan
Organization: School of Life Sciences, Xiamen University
Abstract: The subtracted cDNA libraries for amphioxus gonads had been constructed and a series of genes which are potentially involved in amphioxus gonad development had been identified. On this basis, this study focused on one of these ovary-specific genes AmphiH2A.X and its related genes AmphiH2A-core, found that they had similar gene structures and also encode similar proteins sequences with their vertebrate cognates. For example, AmphiH2A.X protein contains a canonical SQEY (Ser-Gln-Glu-Tyr) structure, the feature found in other H2A.X. In regard to expression pattern, AmphiH2A-core expresses in all examined organs from the adult, the pattern similar to vertebrate H2A genes, however the expression of AmphiH2A.X is mainly detected in ovary and increases as the ovary developing to be mature. Therefore it could be concluded that AmphiH2A-core play conserved roles in both vertebrates and amphioxus, �H2A.X� have evolved a specific function in amphioxus ovary development.�
Key words: genetics; amphioxus; AmphiH2A-core; AmphiH2A.X; ovary development
发表期数: 2012年3月第5期
引用格式: 李光,王秀,刘晓慧,等. 日本文昌鱼(Branchiostoma japonicum) AmphiH2A-core和AmphiH2A.X基因的克隆及其表达分析[J]. 中国科技论文在线精品论文,2012,5(5):456-462.
 
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