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LASIK对兔角膜基质细胞增殖表型及bFGF表达的影响
发表时间：2012-03-15 浏览量：1491 下载量：726
| 全部作者： | 何正蓉，安美文，李晓娜 |
| 作者单位： | 太原理工大学应用力学与生物医学工程研究所 |
| 摘 要： | 目的：观察准分子激光原位角膜磨镶术（laser assisted in situ keratomileusis, LASIK）后兔角膜上皮及基质层中碱性成纤维细胞生长因子（basic fibroblast growth factor, bFGF）表达及角膜基质细胞增殖表型的变化。方法：建立不同切削量的LASIK手术动物模型，采用免疫荧光染色观察角膜上皮及基质层中的bFGF表达；苏木素-伊红（HE）染色观察角膜基质细胞增殖表型的变化。结果：bFGF在上皮及基质层中均有表达，但基质层中荧光强度总体高于上皮层，70%周组基质层荧光强度明显强于上皮层（P<0.05）。HE染色与正常组相比，术后1周角膜细胞个数先减少，术后1月细胞个数增多，同一手术方式术后1月细胞数量显著多于1周（P<0.05）；细胞表型也有较明显的变化。结论：LASIK术后角膜基质细胞增殖表型发生了变化，bFGF的表达主要集中在基质层。� |
| 关 键 词： | 生物医学工程；准分子激光原位角膜磨镶术；角膜基质细胞；增殖；表型；成纤维细胞生长因子 |
| Title： | Effects of LASIK on proliferation phenotype and the bFGF expression of corneal stromal cell |
| Author： | HE Zhengrong, AN Meiwen, LI Xiaona |
| Organization： | Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology |
| Abstract： | Objective: To observe the expression of basic fibroblast growth factor (bFGF) in epithelial layer and stromal layer of cornea, and changes on proliferation phenotype of corneal stromal cell after laser assisted in situ keratomileusis (LASIK）. Methods: Rabbit corneas were established by LASIK surgery with different residual stromal bed. Immunofluorescence staining was used to observe the expression of bFGF in epithelial layer and stromal layer; HE staining was used to observe changes on proliferation phenotype of corneal stromal cell. Results: bFGF is expressed both in epithelial layer and stromal layer, but fluorescence intensity of stromal layer is higher than stromal layer on the whole; and the fluorescence intensity of stromal layer is obviously higher than stromal layer (70% a week, �P<0.05�). Compared with normal group, the number of corneal cell reduces at one week after LASIK surgery, while at one month the number of corneal cell increases, with the same operation method. The number of corneal cell at one month after LASIK surgery is significantly more than that at one week （P<0.05）. The phenotype of corneal cell also changes apparently. Conclusion: Proliferation phenotype of corneal stromal cell changes after LASIK surgery, and the expression of bFGF mainly concentrates on the stromal layer.� |
| Key words： | biomedical engineering; laser assisted in situ keratomileusis; corneal stromal cell; proliferation; phenotype; basic fibroblast growth factor |
| 发表期数： | 2012年3月第5期 |
| 引用格式： | 何正蓉，安美文，李晓娜. LASIK对兔角膜基质细胞增殖表型及bFGF表达的影响[J]. 中国科技论文在线精品论文，2012，5（5）：482-488. |
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