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猪唾液酸粘附素的分段表达及功能鉴定

发表时间:2012-06-15  浏览量:1484  下载量:743
全部作者: 黄恋琦,程彪,黄昆
作者单位: 华中科技大学同济药学院
摘 要: 选取传统的化学交联法、亲和标记法及目前最新的对未修饰蛋白质的光催化交联法(photo- induced cross- linking of unmodified proteins,PICUP)3种常见的蛋白质交联检测方法进行比较,并以实验室中对猪胰岛素和人胰岛淀粉样多肽的研究结果为例,重点阐述了PICUP的特点、原理、适用范围及影响其反应的因素。实验结果显示:PICUP相较其他2种交联法具有实验操作简单、适用条件范围广、耗时短等特点,同时也展望了该方法在生命科学等相关研究领域的发展趋势。
关 键 词: 生物药物学;光催化交联法;综述;交联
Title: Research on the principle and application of a novel photo- induced cross- linking of unmodied proteins
Author: HUANG Lianqi, CHENG Biao, HUANG Kun
Organization: Tongji College of Pharmacy, Huazhong University of Science and Technology
Abstract: In this paper, three methods containing traditional chemical cross- linking, affinity labeling, and newly developed photo- induced cross- linking of unmodified proteins (PICUP) are picked out to be compared. And based on the experiments results on porcine insulin (PI) and human islet amyloid polypeptide (hIAPP) in our laboratory, points of the paper are focused on the principle, application, advantages and disadvantages of the PICUP methodology. According to the results, the new method has some characteristics, such as simple- procedures, wide- range as well as short time and so on. The research progress of the method in bioscience is also discussed in this paper.
Key words: biopharmaceutics; photo- induced cross- linking of unmodified proteins; reviews; cross- linking
发表期数: 2012年6月第11期
引用格式: 黄恋琦,程彪,黄昆. 猪唾液酸粘附素的分段表达及功能鉴定[J]. 中国科技论文在线精品论文,2012,5(11):1011-1017.
 
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