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人巨细胞病毒感染对成纤维细胞分泌RANTES的影响

发表时间:2012-06-15  浏览量:1041  下载量:501
全部作者: 张煈
作者单位: 北京大学第三医院妇产科
摘 要: 目的:研究人巨细胞病毒(human cytomegalovirus,HCMV)对人胚肺成纤维细胞(human embryonic lung fibroblast,HELF)分泌正常T细胞表达和分泌受活化调节的蛋白(regulated on activation, normal T expressed and secreted, RANTES)的影响,以探讨HCMV感染所致免疫病理改变的机制。方法:用RT- PCR法检测RANTES mRNA的表达,用ELISA法测定RANTES蛋白的分泌。结果:HELF细胞感染HCMV后,RANTES mRNA在感染后8 h可测出,24 h达到高峰,其后虽有下降,但仍维持较高水平;RANTES蛋白分泌在感染后24 h达到高峰,随后急剧下降,至72 h基本无表达。结论:HCMV可通过模拟β族趋化因子受体而下调RANTES的分泌,逃避感染时局部免疫应答。
关 键 词: 围产医学;巨细胞病毒;趋化因子类;免疫应答
Title: Effect of human cytomegalovirus infection on secretion of RANTES in the cultured human fibroblasts
Author: ZHANG Yan
Organization: Department of Obstetrics and Gynecology, Third Hospital, Peking University
Abstract: Objective: To investigate the effect of human cytomegalovirus (HCMV) infection on the expression of regulated on activation, normal T expressed and secreted (RANTES) in human embryonic lung fibroblast (HELF) so as to explore the possible mechanism of the immune- pathological changes caused by HCMV infection. Methods: Expression of RANTES mRNA was detected by RT- PCR. Secretion of RANTES protein was quantitated by ELISA. Results: After HCMV infection, the expression of RANTES mRNA was occurred at 8 h.p.i, with a maximum at 24 h. p.i, since then it remained relatively high level. The expression of RANTES protein peaked at 24 h. p.i, then dropped sharply and was almost undetectable by the time at 72 h.p.i. Conclusion: HCMV may down- regulate extra- cellular production of RANTES protein via mimicking the receptor of β family chemokine as a mechanism to evade the local immune response to infection.
Key words: prenatal medicine; cytomegalovirus; chemotactic factors; immune response
发表期数: 2012年6月第11期
引用格式: 张煈. 人巨细胞病毒感染对成纤维细胞分泌RANTES的影响[J]. 中国科技论文在线精品论文,2012,5(11):1035-1038.
 
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