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糖尿病肾病患者podocalyxin及TGF-β1表达的意义

发表时间:2012-06-15  浏览量:973  下载量:437
全部作者: 邢玲玲,于连英,傅淑霞,杨林,李邵梅,刘青娟
作者单位: 河北医科大学第二医院;河北医科大学基础医学院
摘 要: 目的:探讨糖尿病肾病(diabetic nephropathy,DN)患者尿液中足细胞标志蛋白——足盂蛋白(podocalyxin,PCX)的水平与肾组织中转化生长因子- β1(transforming growth factor- β1,TGF- β1)表达的关系。方法:收集30例DN患者24 h尿标本,并以10例健康志愿者24 h尿标本作为对照,ELISA方法检测患者尿液中PCX的水平。DN患者的肾组织标本根据肾小球病变分为3组,并以10例肾脏肿瘤远端肾组织作为对照组。采用免疫组织化学方法检测各组肾小球内TGF- β1的表达。结果:DN患者尿PCX的水平明显高于健康对照组[(32.3±3.5) mg/L vs (2.0±0.4) mg/L,P<0.05],并随肾小球病变加重表达增多。肾小球内TGF- β1的表达在Ⅲ级病变组显著增多,尤其伴有硬化的区域。尿PCX水平与肾小球TGF- β1表达量、24 h尿蛋白排泄量、血肌酐(Scr)呈正相关关系(r分别为0.835,0.742,0.599,均有P<0.01)。结论:尿PCX水平可作为预测DN患者病情的新指标;TGF- β1与DN足细胞损伤有关。
关 键 词: 内科学;足盂蛋白;转化生长因子- β1;糖尿病肾病
Title: Role of podocalyxin and TGF-β1 in patients with diabetic nephropathy
Author: XING Lingling, YU Lianying, FU Shuxia, YANG Lin, LI Shaomei, LIU Qingjuan
Organization: The Second Hospital of Hebei Medical University; Basic College of Hebei Medical University
Abstract: Objective: To investigate the relation between podocyte marker protein podocalyxin (PCX) and transforming growth factor- β1 (TGF- β1) in patients with diabetic nephropathy (DN). Methods: Twenty- four hour urinary samples from 30 patients with DN and 10 healthy volunteers as control group were collected to detect the level of PCX by ELASA. All patients with DN were divided into three groups by glomerular lesion and 10 cases of renal tumors distal renal tissue as control group. The expression of TGF- β1 in glomeruli was detected by immunohistochemistry. Results: The levels of PCX were significantly higher in the urine from patients with DN compared with those in the control group [(32.3±3.5) mg/L vs (2.0±0.4) mg/L, P<0.05), and gradually increased along with glomerular lesion aggravating. The expression of TGF- β1 in glomeruli was obvious in the third grade glomerular lesion, especially in sclerotic area. There were obviously positive correlation between the level of urine PCX and expression of TGF- β1 in diabetic glomeruli, 24h urine protein excretion, serum creatinine (r=0.835 P<0.01, r=0.742 P<0.01, r=0.599 P<0.01, respectively). Conclusion: The level of urine PCX can be used as a novel marker for forecasting the condition of patients with DN and TGF- β1 correlates with the podocyte injury.
Key words: medicine; podocalyxin; transforming growth factor- β1; diabetic nephropathy
发表期数: 2012年6月第11期
引用格式: 邢玲玲,于连英,傅淑霞,等. 糖尿病肾病患者podocalyxin及TGF-β1表达的意义[J]. 中国科技论文在线精品论文,2012,5(11):1065-1069.
 
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