您的位置:首页  > 论文页面

gga-miR-92对靶基因ATRX的调控分析

发表时间:2012-06-15  浏览量:1226  下载量:570
全部作者: 王晓,苗楠,龚萍,彭秀丽,龚炎长,俸艳萍
作者单位: 华中农业大学农业动物遗传育种与繁殖教育部重点实验室
摘 要: 为研究gga- miR- 92在鸡性腺发育中的调控作用,首先通过生物信息学预测与性腺发育相关的靶基因,然后采用双荧光素酶报告基因法在鸡DF- 1细胞中验证miR- 92对靶基因的调控作用。结果表明:TargetScan和miRDB 2个软件预测到X连锁的α地中海贫血/智力障碍综合症(alpha thalassemia/mental retardation syndrome X- linked,ATRX)是gga- miR- 92的靶基因,成功克隆并构建了ATRX 3′UTR的表达质粒,双荧光素酶报告基因分析表明miR- 92能显著抑制融合ATRX 3′UTR报告基因的荧光表达量(P<0.01)。研究结果证明了ATRX与gga- miR- 92间存在负向调控关系。
关 键 词: 养禽学;鸡;miR- 92;X连锁的α地中海贫血/智力障碍综合症;性腺发育
Title: Analysis on regulation of gga-miR-92 on target gene ATRX
Author: WANG Xiao, MIAO Nan, GONG Ping, PENG Xiuli, GONG Yanchang, FENG Yanping
Organization: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University
Abstract: To study the regulation effect of gga- miR- 92 on chicken gonadal development, targets related to gonadal development were first predicted by using bioinformatics methods, then the regulatory relation of miR- 92 with target was identified by dual luciferase reporter assay in chicken DF- 1 cell. The results showed that alpha thalassemia/mental retardation syndrome X- linked (ATRX) was predicted to be a target of gga- miR- 92, and recombined expression vectors of ATRX 3′- UTR were constructed successfully. Dual luciferase reporter assay showed that miR- 92 decreased the activity of luciferase co- transfected with ATRX vector. It can be concluded that ATRX might be negatively regulated by gga- miR- 92.
Key words: poultry science; chicken; miR- 92; alpha thalassemia/mental retardation syndrome X- linked; gonadal development
发表期数: 2012年6月第11期
引用格式: 王晓,苗楠,龚萍,等. gga-miR-92对靶基因ATRX的调控分析[J]. 中国科技论文在线精品论文,2012,5(11):1070-1074.
 
0 评论数 0
暂无评论
友情链接