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中华补血草(Limonium sinense)潮霉素敏感浓度的确定

发表时间:2012-09-15  浏览量:996  下载量:337
全部作者: 张小通,李燕,曹子谊
作者单位: 山东师范大学生命科学学院
摘 要: 目的:以野生型中华补血草为材料,确定其对抗生素潮霉素(hygromycin)的敏感浓度,为之后以潮霉素为基础的基因工程载体构建及转基因植株筛选提供基础。方法:以补血草叶片为材料,消毒后经叶盘法切片后,培养于添加不同浓度潮霉素的BI培养基中30 d,研究其对不定芽的影响。结果与结论:不定芽对潮霉素的敏感浓度为20 mg/L.
关 键 词: 植物遗传学;中华补血草;组织培养;潮霉素
Title: Determination of sensitivity of Limonium sinense to antibiotics hygromycin
Author: ZHANG Xiaotong, LI Yan, CAO Ziyi
Organization: School of Life Sciences, Shandong Normal University
Abstract: Objective: To determine the sensitive concentration of Limonium sinense to hygromycin, so as to basis for further genetic engineering carrier construction taking hygromycin as foundation and screening of transgenic plants. Methods: Leaves of L. sinense were disinfected and cut into pieces and transferred to adventitious BI media added with different concentrations of hygromycin for 30 days to research the influence on adventitious buds. Results and conclusion: The result showed that concentration of antibiotics hygromycin which had influences on adventitious buds induction and growth was 20 mg/L.
Key words: plant genetics; Limonium sinense; tissue culture; hygromycin
发表期数: 2012年9月第17期
引用格式: 张小通,李燕,曹子谊. 中华补血草(Limonium sinense)潮霉素敏感浓度的确定[J]. 中国科技论文在线精品论文,2012,5(17):1597-1600.
 
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