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冬枣ACC氧化酶基因的选择性剪接

发表时间:2012-09-15  浏览量:961  下载量:309
全部作者: 林星谷,庞晓明
作者单位: 北京林业大学林木育种国家工程实验室;北京林业大学林木花卉遗传育种教育部重点实验室;北京林业大学计算生物学中心
摘 要: 以冬枣(Ziziphus jujuba)为研究对象,从cDNA文库中获得了与ZjACO1同源性很高的P-ZjACO基因,其开放阅读框全长138 bp,编码45个氨基酸。分别以冬枣cDNA和DNA为模板进行基因片段克隆,测序结果分析证实冬枣的ACC氧化酶基因存在选择性剪接,并且不是单一的选择性剪接类型,而是同属于剪接缺失外显子和剪接保留内含子类型。
关 键 词: 分子生物学;ACC氧化酶;选择性剪接;克隆;冬枣
Title: Alternative splicing of ACC oxidase gene in Ziziphus jujuba
Author: LIN Xinggu, PANG Xiaoming
Organization: National Engineering Laboratory for Tree Breeding, Beijing Forestry University; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University; Centre for Computational Biology, Beijing Forestry University
Abstract: In this paper, P-ZjACO gene homologous with ZjACO1 is obtained from the Ziziphus jujuba cDNA library. The ORF of P-ZjACO gene is 138 bp, which encodes 45 amino acids. The gene fragment is respectively cloned from the template of Z. jujuba cDNA and DNA. Sequencing analysis proves that, there is alternative splicing in the ACC oxidase gene of Z. jujuba, which is not a single type of alternative splicing, but belongs to the types of missing exon splicing and intron retention splicing.
Key words: molecular biology; ACC oxidase; alternative splicing; cloning; Ziziphus jujube
发表期数: 2012年9月第17期
引用格式: 林星谷,庞晓明. 冬枣ACC氧化酶基因的选择性剪接[J]. 中国科技论文在线精品论文,2012,5(17):1635-1642.
 
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