您的位置:首页  > 论文页面

肠道特异性TR3过表达转基因小鼠模型的构建与检测

发表时间:2012-09-15  浏览量:1228  下载量:399
全部作者: 刘庆锋,杨萌,骆启聪,李博安
作者单位: 厦门大学生命科学学院;厦门大学附属第一医院中心实验室
摘 要: 目的:为将来研究孤儿受体TR3在肠道组织发育和在结直肠癌发生发展中的功能,构建在Villin启动子作用下肠道上皮组织特异性过表达外源TR3基因的小鼠模型。方法:构建pVillin-flag-TR3的转基因小鼠,并通过反转录PCR(reverse transcription-PCR, RT-PCR)、蛋白质印迹实验(Western blotting)和免疫组织化学(immunohistochemistry,IHC)染色3种不同的方法检测转基因片段的表达。结果与结论:成功构建了肠道特异性TR3过表达的转基因小鼠模型,并在转基因小鼠的肠道上皮组织中检测到flag-TR3转基因片段的表达。
关 键 词: 细胞生物学;TR3;转基因小鼠模型;肠道上皮组织
Title: Construction and detection of intestinal-specific TR3 overexpression transgenic mice modc
Author: LIU Qingfeng, YANG Meng, LUO Qicong, LI Boan
Organization: School of Life Sciences, Xiamen University; The Central Laboratory of The First Affiliated Hospital of Xiamen University
Abstract: Objective: In order to investigate the function of orphan receptor TR3 in the development of intestine and in the progression of colorectal cancer, this subject successfully constructed intestinal epithelial tissue-specific TR3 overexpression mouse model under the control of Villin promoter. Methods: The pVillin-flag-TR3 transgenic mice were constructed and the expression of the transgene fragment was detected by three different methods (RT-PCR, Western blotting and IHC). Results and conclusion: The intestinal specific TR3 overexpression transgenic mice model were successfully constructed and the expression of flag-TR3 transgenic fragment was detected in the intestinal epithelium of transgenic mice.
Key words: cellular biology; TR3; transgenic mouse model; intestinal epithelium
发表期数: 2012年9月第17期
引用格式: 刘庆锋,杨萌,骆启聪,等. 肠道特异性TR3过表达转基因小鼠模型的构建与检测[J]. 中国科技论文在线精品论文,2012,5(17):1661-1666.
 
0 评论数 0
暂无评论
友情链接