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加压CO2对牛乳中主要腐败菌及致病菌的杀菌效果

发表时间:2012-10-15  浏览量:1214  下载量:330
全部作者: 姚春艳,刘美凤,付瑞东,李晓东
作者单位: 东北农业大学食品学院,乳品科学教育部重点实验室;黑龙江职业学院食品工程学院
摘 要: 研究了加压CO2对牛乳中主要腐败菌荧光假单胞菌(Pseudomonas fluorescent)、大肠杆菌(Escherichia coli)和主要致病菌金黄色葡萄球菌(Staphylococcus aureus)的杀菌效果。通过改变CO2压力、加压时间、加压温度和加压介质研究了影响杀菌效果的因素。结果表明:增加压力、延长加压时间和提高加压温度都可以增强加压CO2对牛乳中3种菌的杀菌效果;革兰氏阴性菌比革兰氏阳性菌对加压CO2处理更加敏感;在灭菌牛乳中接种单一细菌时,在45℃下使用6 MPa CO2加压40 min可以使大肠杆菌和荧光假单胞菌的杀菌率达到99.09%和99.23%,对金黄色葡萄球菌杀菌率为91.81%;牛乳成分对细菌具有保护作用。�
关 键 词: 乳加工技术;加压CO2;杀菌效果;牛乳;腐败菌;致病菌�
Title: Sterilization effect of pressurized CO2 on major spoilage and pathogenic bacteria in milk
Author: YAO Chunyan, LIU Meifeng, FU Ruidong, LI Xiaodong
Organization: Key Laboratory of Dairy Science, Ministry of Education, Food College of Northeast Agricultural University; School of Food Engineering, Heilongjiang Polytechnic
Abstract: This paper stuided studied effect of pressurized CO2 on major spoilage (Pseudomonas fluorescent, Escherichia coli) and pathogenic bacteria (Staphylococcus aureus) in milk. The effects of CO2 pressure, treatment time, treatment temperature and treatment media on sterilization efficiency were investigated. The results indicated that the effect on three bacterium was increased by increasing pressure, treatment time and treatment temperature. Gram-negative bacteria were more sensitive than gram-positive bacteria to the treatment of pressurized CO2. When single bacterium was inoculated in sterilized milk, sterilizing ratio of pressurized CO2 were 99.09% for E. coli, 99.23% for P. fluorescent and 91.81% for S. aureus respectively, under 45℃, 6 MPa, 40 min. The bacterium was protected by milk protein and fat.�
Key words: dairy process technology; pressurized CO2; sterilization effects; milk; spoilage bacteria; pathogenic bacteria
发表期数: 2012年10月第19期
引用格式: 姚春艳,刘美凤,付瑞东,等. 加压CO2对牛乳中主要腐败菌及致病菌的杀菌效果[J]. 中国科技论文在线精品论文,2012,5(19):1799-1804.
 
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