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杠柳杀虫活性成分杠柳毒素NW对东方粘虫肌细胞内钙浓度的影响

发表时间:2012-10-15  浏览量:1111  下载量:311
全部作者: 胡兆农,廉喜红,刘健,吴文君
作者单位: 西北农林科技大学植物保护学院,陕西省植物源农药研究与开发重点实验室
摘 要: 为探索植物源杀虫活性成分杠柳毒素NW(periplocoside NW,PSNW)的作用机理,采用激光共聚焦显微技术结合荧光探针标记,研究PSNW对东方粘虫(Mythimna separata)成虫肌细胞内[Ca2+]的影响。结果表明:PSNW在较高浓度(μmol/L~mmol/L水平)时导致肌细胞内[Ca2+]降低,达不到肌肉收缩所需的[Ca2+],致使昆虫肌肉不能收缩,软瘫麻痹;在较低浓度(nmol/L水平)时则导致肌细胞内[Ca2+]升高,致使昆虫肌肉挛缩,僵直麻痹。研究结果说明肌细胞内Ca2+通道可能是PSNW的作用靶标之一。�
关 键 词: 农药学;杀虫植物;杠柳毒素NW;东方粘虫;胞内Ca2+通道�
Title: Effects of periplocoside NW from Periploca sepium on intracellar[Ca2+] in of adults of Mythimna separata muscle cells
Author: HU Zhaonong, LIAN Xihong, LIU Jian, WU Wenjun
Organization: Shaanxi Province Key Laboratory of Research & Development on Botanical Pesticide, College of Plant Protection, Northwest A&F University
Abstract: To understand the insecticidal mechanism of periplocoside NW (PSNW) isolated from Periploca sepium, the effects of PSNW on the muscle intracellular [Ca2+] of Mythimna separata adults were determined by means of laser confocal microscope together with calcium fluorescent indicator. The results showed that the intracellular [Ca2+] decreased when treated with high concentration (μmol/L~mmol/L level) of PSNW, while the intracellular [Ca2+] raised when treated with low concentration (nmol/L level) of PSNW. These results suggested that the intracellular calcium channel of muscle cell might be one of the action targets of PSNW.�
Key words: pesticide science; botanical insecticide; periplocoside NW; Mythimna separata; intracellular Ca2+ channel
发表期数: 2012年10月第19期
引用格式: 胡兆农,廉喜红,刘健,等. 杠柳杀虫活性成分杠柳毒素NW对东方粘虫肌细胞内钙浓度的影响[J]. 中国科技论文在线精品论文,2012,5(19):1858-1863.
 
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