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杠柳杀虫活性成分杠柳毒素NW对东方粘虫肌细胞内钙浓度的影响
发表时间：2012-10-15 浏览量：1390 下载量：380
| 全部作者： | 胡兆农，廉喜红，刘健，吴文君 |
| 作者单位： | 西北农林科技大学植物保护学院，陕西省植物源农药研究与开发重点实验室 |
| 摘 要： | 为探索植物源杀虫活性成分杠柳毒素NW（periplocoside NW，PSNW）的作用机理，采用激光共聚焦显微技术结合荧光探针标记，研究PSNW对东方粘虫（Mythimna separata）成虫肌细胞内［Ca2+］的影响。结果表明：PSNW在较高浓度（μmol/L～mmol/L水平）时导致肌细胞内［Ca2+］降低，达不到肌肉收缩所需的［Ca2+］，致使昆虫肌肉不能收缩，软瘫麻痹；在较低浓度（nmol/L水平）时则导致肌细胞内［Ca2+］升高，致使昆虫肌肉挛缩，僵直麻痹。研究结果说明肌细胞内Ca2+通道可能是PSNW的作用靶标之一。� |
| 关 键 词： | 农药学；杀虫植物；杠柳毒素NW；东方粘虫；胞内Ca2+通道� |
| Title： | Effects of periplocoside NW from Periploca sepium on intracellar［Ca2+］ in of adults of Mythimna separata muscle cells |
| Author： | HU Zhaonong, LIAN Xihong, LIU Jian, WU Wenjun |
| Organization： | Shaanxi Province Key Laboratory of Research & Development on Botanical Pesticide, College of Plant Protection, Northwest A&F University |
| Abstract： | To understand the insecticidal mechanism of periplocoside NW (PSNW) isolated from Periploca sepium, the effects of PSNW on the muscle intracellular ［Ca2+］ of Mythimna separata adults were determined by means of laser confocal microscope together with calcium fluorescent indicator. The results showed that the intracellular ［Ca2+］ decreased when treated with high concentration (μmol/L~mmol/L level) of PSNW, while the intracellular ［Ca2+］ raised when treated with low concentration (nmol/L level) of PSNW. These results suggested that the intracellular calcium channel of muscle cell might be one of the action targets of PSNW.� |
| Key words： | pesticide science; botanical insecticide; periplocoside NW; Mythimna separata; intracellular Ca2+ channel |
| 发表期数： | 2012年10月第19期 |
| 引用格式： | 胡兆农，廉喜红，刘健，等. 杠柳杀虫活性成分杠柳毒素NW对东方粘虫肌细胞内钙浓度的影响[J]. 中国科技论文在线精品论文，2012，5（19）：1858-1863. |
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