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细胞因子信号传导抑制蛋白1对糖尿病小鼠肾损伤作用的实验研究

发表时间:2012-12-15  浏览量:1204  下载量:378
全部作者: 刘青娟,邢玲玲,刘淑霞,段惠军
作者单位: 河北医科大学基础医学院;河北医科大学第二医院肾内科
摘 要: 目的:探讨细胞因子信号传导抑制蛋白1(suppressor of cytokine signaling 1,SOCS-1)在糖尿病小鼠肾组织炎性损伤中的作用。方法:雄性CD-1小鼠随机分为4组,分别为对照组、糖尿病组、SOCS-1质粒转染组及空载体转染组。腹腔注射链脲佐菌素(streptozotocin,STZ)诱发糖尿病小鼠模型,转染组在成模后给予尾静脉注射SOCS-1质粒或空质粒载体,每隔7 d注射一次。4周后收集标本,检测各组动物的血糖、24 h 尿蛋白;蛋白印迹法(Western blotting)检测肾组织中SOCS-1蛋白的水平。流式细胞术检测肾组织中巨噬细胞标志蛋白CD68(cluster of differentiation 68)的水平;ELISA检测肾组织中白细胞介素-1β(interleukin-1β,IL-1β)和转化生长因子-β1(transforming growth factor-β1,TGF-β1)的水平。结果:过表达SOCS-1能抑制糖尿病小鼠肾组织中CD68,IL-1β和TGF-β1的表达,降低糖尿病小鼠尿蛋白排泄,但对血糖无影响。结论:过表达SOCS-1能通过减轻糖尿病小鼠肾组织炎症反应而缓解肾损伤。
关 键 词: 病理学;糖尿病肾病;细胞因子信号传导抑制蛋白1;炎症;白细胞介素-1β
Title: Experimental study on the role of SOCS-1 on renal injury in diabetic mice
Author: LIU Qingjuan, XING Lingling, LIU Shuxia, DUAN Huijun
Organization: School of Basic Medical Sciences, Hebei Medical University; Department of Nephrology, The Second Hospital of Hebei Medical University
Abstract: Objective: To investigate the role of suppressor of cytokine signaling 1 (SOCS-1) on renal injury in diabetic mice. Methods: Male CD-1 mice were randomly divided into 4 groups: control group, diabetic group, empty plasmid vector transfection group and SOCS-1 plasmid transfection group. Mice were given an intraperitoneal injection of streptozotocin (STZ) to induce the model of diabetic mice. Then transfection mice were given an caudal vein injection of SOCS-1 plasmid or empty vector once 7 day. After 4 weeks of injection, specimens were collected to detect the blood glucose and 24 h urine protein. Western blotting analysis were used to determine the expression level of SOCS-1 protein. The expression level of macrophage marker protein CD68 in renal tissue was detected by flow cytometry. The levels of interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) in renal tissue were detected by ELISA. Results: Overexpression of SOCS-1 could inhibit the expression of cluster of differentiation 68 (CD68), IL-1β and TGF-β1 in renal tissue and ameliorate the excretion of urine protein of diabetic mice, but it had no effects on blood glucose level. Conclusion: Overexpression of SOCS-1 could relieve renal injury through reducing the inflammatory response in renal tissue of diabetic mice.
Key words: pathology; diabetic nephropathy; suppressor of cytokine signaling 1; inflammation; interleukin-1β
发表期数: 2012年12月第23期
引用格式: 刘青娟,邢玲玲,刘淑霞,等. 细胞因子信号传导抑制蛋白1对糖尿病小鼠肾损伤作用的实验研究[J]. 中国科技论文在线精品论文,2012,5(23):2221-2225.
 
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