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细胞因子信号传导抑制蛋白1对糖尿病小鼠肾损伤作用的实验研究
发表时间：2012-12-15 浏览量：1723 下载量：495
| 全部作者： | 刘青娟，邢玲玲，刘淑霞，段惠军 |
| 作者单位： | 河北医科大学基础医学院；河北医科大学第二医院肾内科 |
| 摘 要： | 目的：探讨细胞因子信号传导抑制蛋白1（suppressor of cytokine signaling 1，SOCS-1）在糖尿病小鼠肾组织炎性损伤中的作用。方法：雄性CD-1小鼠随机分为4组，分别为对照组、糖尿病组、SOCS-1质粒转染组及空载体转染组。腹腔注射链脲佐菌素（streptozotocin，STZ）诱发糖尿病小鼠模型，转染组在成模后给予尾静脉注射SOCS-1质粒或空质粒载体，每隔7 d注射一次。4周后收集标本，检测各组动物的血糖、24 h 尿蛋白；蛋白印迹法（Western blotting）检测肾组织中SOCS-1蛋白的水平。流式细胞术检测肾组织中巨噬细胞标志蛋白CD68（cluster of differentiation 68）的水平；ELISA检测肾组织中白细胞介素-1β（interleukin-1β，IL-1β）和转化生长因子-β1（transforming growth factor-β1，TGF-β1）的水平。结果：过表达SOCS-1能抑制糖尿病小鼠肾组织中CD68，IL-1β和TGF-β1的表达，降低糖尿病小鼠尿蛋白排泄，但对血糖无影响。结论：过表达SOCS-1能通过减轻糖尿病小鼠肾组织炎症反应而缓解肾损伤。 |
| 关 键 词： | 病理学；糖尿病肾病；细胞因子信号传导抑制蛋白1；炎症；白细胞介素-1β |
| Title： | Experimental study on the role of SOCS-1 on renal injury in diabetic mice |
| Author： | LIU Qingjuan, XING Lingling, LIU Shuxia, DUAN Huijun |
| Organization： | School of Basic Medical Sciences, Hebei Medical University; Department of Nephrology, The Second Hospital of Hebei Medical University |
| Abstract： | Objective: To investigate the role of suppressor of cytokine signaling 1 (SOCS-1) on renal injury in diabetic mice. Methods: Male CD-1 mice were randomly divided into 4 groups: control group, diabetic group, empty plasmid vector transfection group and SOCS-1 plasmid transfection group. Mice were given an intraperitoneal injection of streptozotocin (STZ) to induce the model of diabetic mice. Then transfection mice were given an caudal vein injection of SOCS-1 plasmid or empty vector once 7 day. After 4 weeks of injection, specimens were collected to detect the blood glucose and 24 h urine protein. Western blotting analysis were used to determine the expression level of SOCS-1 protein. The expression level of macrophage marker protein CD68 in renal tissue was detected by flow cytometry. The levels of interleukin-1β (IL-1β) and transforming growth factor-β1 (TGF-β1) in renal tissue were detected by ELISA. Results: Overexpression of SOCS-1 could inhibit the expression of cluster of differentiation 68 (CD68), IL-1β and TGF-β1 in renal tissue and ameliorate the excretion of urine protein of diabetic mice, but it had no effects on blood glucose level. Conclusion: Overexpression of SOCS-1 could relieve renal injury through reducing the inflammatory response in renal tissue of diabetic mice. |
| Key words： | pathology; diabetic nephropathy; suppressor of cytokine signaling 1; inflammation; interleukin-1β |
| 发表期数： | 2012年12月第23期 |
| 引用格式： | 刘青娟，邢玲玲，刘淑霞，等. 细胞因子信号传导抑制蛋白1对糖尿病小鼠肾损伤作用的实验研究[J]. 中国科技论文在线精品论文，2012，5（23）：2221-2225. |
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