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短期化疗对乳腺癌组织UCH-L1表达的影响

发表时间:2012-12-15  浏览量:1265  下载量:486
全部作者: 包芸,王文娟,李琼,陈琦,刘秀萍,许祖德,唐峰
作者单位: 复旦大学附属华山医院病理科;复旦大学上海医学院病理学系
摘 要: 目的:探讨乳腺癌患者术前短期化疗对肿瘤细胞泛素羧基末端水解酶1(ubiquitin carboxy-terminal hydrolase L1,UCH-L1)、表皮生长因子受体(epithelial growth factor receptor,EGFR)及P-糖蛋白(P-glycoprotein,P-gp)表达的影响。方法:采用免疫组化(EnVision法)检测40例乳腺浸润性导管癌患者多药耐药(multidrug resistance,MDR)药物治疗前后肿瘤细胞内UCH-L1,EGFR和P-gp的表达。结果:免疫组化染色显示,化疗后肿瘤细胞UCH-L1的表达较化疗前降低(P<0.05),而EGFR的阳性率明显高于化疗前(P<0.01);化疗后P-gp的阳性率与化疗前无显著差异(P>0.05);3个标记结果与肿瘤体积缩小和淋巴结转移均无明显相关性。结论:短期MDR药物化疗可以杀伤肿瘤细胞,也可导致UCH-L1,EGFR和P-gp表达的变化,其机制可能在于化疗药物对肿瘤细胞的选择性损伤,但未诱发细胞外基质金属蛋白酶诱导者CD147等下游因子的表达。
关 键 词: 病理学;乳腺癌;多药耐药药物;泛素羧基末端水解酶1;表皮生长因子受体;P-糖蛋白
Title: Effect of short-term chemotherapy on the expression of UCH-L1 in breast carcinoma
Author: BAO Yun, WANG Wenjuan, LI Qiong, CHEN Qi, LIU Xiuping, XU Zude, TANG Feng
Organization: Depertment of Pathology, Huashan Hospital, Fudan University; Department of Pathology, Shanghai Medical College, Fudan University
Abstract: Objective: To observe the effects of short-term chemotherapy on the expression of ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), epithelial growth factor receptor (EGFR) and P-glycoprotein (P-gp) in the breast carcinoma before surgery. Methods: The expressions of UCH-L1, EGFR and P-gp were assessed by immunohistochemistry (EnVision method) in 40 cases breast carcinoma before and after treatment by multidrug resistance (MDR) drugs. Results: The expression of UCH-L1 was decreased after chemotherapy with MDR drugs when comparing with that before chemotherapy (P<0.05), while the rate of positive EGFR expression increased significantly after chemotherapy (P<0.01). However, the expression of P-gp had no significant difference before and after chemotherapy (P>0.05). Negative associations were revealed between UCH-L1, EGFR and P-gp expression and volume reduce of tumor and lymph node metastasis. Conclusion: Short-term chemotherapy could kill tumor cells and influence the expression of UCH-L1, EGFR and P-gp. The mechanisms may be the selectivity damages of the MDR drugs on the tumor cells. However, the expression of CD147 and other downstream factors had no changes after chemotherapy with MDR drugs.
Key words: pathology; breast carcinoma; multidrug resistance drug; ubiquitin carboxy-terminal hydrolase L1; epithelial growth factor receptor; P-glycoprotein
发表期数: 2012年12月第23期
引用格式: 包芸,王文娟,李琼,等. 短期化疗对乳腺癌组织UCH-L1表达的影响[J]. 中国科技论文在线精品论文,2012,5(23):2226-2230.
 
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