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中枢肿瘤坏死因子-α对糖尿病大鼠交感神经活动的影响

发表时间:2012-12-15  浏览量:1124  下载量:340
全部作者: 任晓霞,宋新爱,许艳艳,齐杰,苏青,索玉平,康玉明
作者单位: 山西医科大学基础医学院;西安交通大学医学院;山西省人民医院妇产科
摘 要: 研究旨在探讨中枢肿瘤坏死因子-α(tumour necrosis factor-α, TNF-α)及其合成阻断剂己酮可可碱(pentoxifylline,PTX)对糖尿病(diabete mellitus, DM)大鼠交感神经活动的影响。实验通过将健康雄性SD(sprage-dawley)大鼠随机分为糖尿病干预组(DM+PTX)、糖尿病对照组(DM+VEH)、正常干预组(SHAM+PTX)和正常对照组(SHAM+VEH),并采用高脂高糖饲料喂养4周,加链脲佐菌素(streptozotocin, STZ)注射建立糖尿病大鼠模型。干预组通过侧脑室插管给予PTX,对照组给予人工脑脊液(vehicle,VEH)。4周后对各组大鼠分别进行体重、血糖测量。应用免疫组化检测大鼠中枢下丘脑室旁核(paraventricular nucleus,PVN)内TNF-α的表达,并运用电极记录肾交感神经放电(renal sympathetic nerve activity,RSNA)情况。所得结果与SHAM 组比较,得出如下结论:1) DM组大鼠PVN内TNF-α表达增加,且DM+SHAM组高于DM+PTX组;2) DM组大鼠肾交感神经放电明显增强,DM+PTX组与DM+SHAM组比较,肾交感神经放电减弱。阻断中枢PVN区TNF-α合成,可以明显降低糖尿病时交感神经兴奋性。研究初步证明中枢TNF-α对糖尿病大鼠交感神经活动存在重要的影响。
关 键 词: 病理学;交感神经;肿瘤坏死因子-α;糖尿病;下丘脑室旁核
Title: Effects of brain tumour necrosis factor-α on sympathetic nerve activity in diabetic rats
Author: REN Xiaoxia, SONG Xin’ai, XU Yanyan, QI Jie, SU Qing, SUO Yuping, KANG Yuming
Organization: Preclinical Medical College, Shanxi Medical University; College of Medicine, Xi’an Jiaotong University; Department of Gynecology and Obstetrics, Shanxi Provincial People’s Hospital
Abstract: The present study was undertaken to investigate the effect of brain tumour necrosis factor-α (TNF-α) and the cytokine synthesis inhibitor pentoxifylline (PTX) on the sympathetic nerve activity in diabete mellitus (DM) rats. Sprage-dawley (SD) rats were randomly divided into SHAM+VEH, SHAM+PTX, DM+VEH and DM+PTX groups. Rats were fed with high fat and sugar diet for 4 weeks following by injection of streptozotocin streptozotocin (STZ) to induce the diabetes mellitus. Rats were implanted with paraventricular nucleus (PVN) cannulae and treated with PTX, or vehicle (VEH) for 4 weeks. After 4-week treatment, the level of blood glucose and body weight were measured. Immunohistochemisty were used to detect the expression of TNF-α in the PVN of hypothalamus. The activity of renal sympathetic nerve activity (RSNA) was recorded using electrodes. The results were: 1) The expression of TNF-α in the PVN of DM group increased, and the level of TNF-α in DM+SHAM group was higher than that in DM+PTX groups; 2) RSNA significantly increased in DM group. Compared with SHAM groups, diabetic rats had augmented RSNA. Sympathetic nerve activity significantly decreased in diabetic rats by blocking synthesis of TNF-α in PVN. In conclusion, the results suggested that TNF-α in the PVN played an important role in sympathetic nerve activity of diabetic rats.
Key words: pathology; sympathetic nerve; tumour necrosis factor-α; diabete mellitus; paraventricular nucleus of hypothalamus
发表期数: 2012年12月第23期
引用格式: 任晓霞,宋新爱,许艳艳,等. 中枢肿瘤坏死因子-α对糖尿病大鼠交感神经活动的影响[J]. 中国科技论文在线精品论文,2012,5(23):2231-2237.
 
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