您的位置:首页  > 论文页面

鸭儿芹提取物抗氧化和保肝作用研究

发表时间:2012-12-15  浏览量:1157  下载量:349
全部作者: 李娟,刘安韬,谢运昌,蒋小华,宁德生
作者单位: 中国科学院广西植物研究所;暨南大学中药及天然药物研究所,中药药效物质基础及创新药物研究广东省高校重点实验室;广西卫生职业技术学院药学系
摘 要: 目的:研究鸭儿芹(Cryptotaenia japonica)提取物的体外抗氧化和对小鼠急性肝损伤的保护作用。方法:通过测定鸭儿芹提取物清除羟自由基、超氧阴离子自由基活力及总抗氧化活力,评价其体外抗氧化作用,采用四氯化碳(CCl4)和酒精诱导小鼠急性肝损伤,测定小鼠血清天门冬氨酸转氨酶(aspartate aminotransferase,AST)和丙氨酸转氨酶(alanine transarninase,ALT),同时测定肝组织中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)抗氧化指标以评价体内保肝作用。结果:鸭儿芹总提取物和正丁醇提取物高剂量有较好的抗氧化作用,并且能降低急性肝损伤小鼠血清AST和ALT含量,且降低肝组织中的MDA含量,升高肝组织中SOD含量。结论:鸭儿芹提取物具有抗氧化和保肝作用,且其抗氧化和清除自由基作用是其保肝作用机制之一。
关 键 词: 中药学;抗氧化;急性肝损伤;鸭儿芹
Title: Hepatoprotective effect and antioxidant activity of Cryptotaenia japonica extracts
Author: LI Juan, LIU Antao, XIE Yunchang, JIANG Xiaohua, NING Desheng
Organization: Guangxi Insitute of Botany, The Chinese Academy of Science; Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, Jinan University; Department of Pharmacy, Guangxi Health College
Abstract: Objective: To study hepatoprotective effect and antioxidant activity of the extracts of Cryptotaenia japonica. Methods: The antioxidant activity of the extracts of C. japonica was evaluated through detecting hydroxy radical, superoxide anion radical and total antioxidant activity in vitro. The mouse model of acute hepatic injury was established by giving CCl4 and alcohol. The activity of aspartate aminotransferase (AST) and alanine transarninase (ALT) in serum and the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in liver tissue were examined to evaluate the hepatoprotective of the extracts in vivo. Results: The high level total extracts and n-butanol extracts of C. japonica had good antioxidant activity in vitro. Furthermore, they could reduce the activities of ALT and AST in serum and the levels of MDA in liver tissue, while could raise the levels of SOD in liver tissue. Conclusion: The results showed that the extracts of C. japonica had a good hepatoprotective effect in acute liver injury. The mechanisms may be related to its effects of antioxidant and scavenging hydroxy radical activity.
Key words: pharmacology; antioxidant; acute liver injury; Cryptotaenia japonica.
发表期数: 2012年12月第23期
引用格式: 李娟,刘安韬,谢运昌,等. 鸭儿芹提取物抗氧化和保肝作用研究[J]. 中国科技论文在线精品论文,2012,5(23):2264-2267.
 
0 评论数 0
暂无评论
友情链接