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丹参注射液联合透明质酸钠关节腔内注射治疗大鼠膝骨关节炎的实验研究

发表时间:2012-12-15  浏览量:1057  下载量:319
全部作者: 尹明,曲良烨,贺荔枝,熊辉
作者单位: 湖南中医药大学继续教育学院
摘 要: 目的:探讨用丹参注射液结合透明质酸钠(sodium hyaluronate,SH)膝关节腔内注射治疗膝骨关节炎(knee osteroarthritis,KOA)的疗效。方法:30只SD(sparague-dawley)大鼠随机分为正常组(A)、骨性关节炎(osteoarthritis,OA)模型组(B)和实验组(C),每组10只。B,C两组共20只大鼠行膝关节腔内注射,分别在第1,3,7天注射4%木瓜蛋白酶溶液与0.03 mol/L的L-半胱氨酸溶液混合液20 μL,以建立KOA动物模型;C组自造模时开始关节内注射SH 0.06 mL和丹参注射液0.06 mL,每周1次。首次注射后第4周测量大鼠膝关节活动范围并处死实验动物,切取膝关节软骨做大体观察及组织病理学观察,进行Mankin’s评分。结果:实验组膝关节活动范围明显大于模型组(P<0.01),软骨破坏减轻,Mankin’s评分有明显改善(P<0.05),两组比较差异有显著性意义。结论:丹参注射液结合SH能改善SD大鼠的关节活动范围,维护软骨的正常建构,减轻软骨退变程度,缓解对软骨细胞的破坏,延缓KOA发展,但不能从根本上阻止KOA的发展。
关 键 词: 中医学;丹参注射液;透明质酸钠;膝骨性关节炎;大鼠;实验研究
Title: Experimental study on intra-articular injection of danshen and sodium hyaluronate for the treatment of rat osteoarthritis
Author: YIN Ming, QU Liangye, HE Lizhi, XIONG Hui
Organization: School of Continuing Education, Hunan Univesity of Traditional Chinese Medicine
Abstract: Objective: To observe the effects of intra-articular injection of danshen and sodium hyaluronate (SH) on treatment of rat knee osteroarthritis (KOA). Methods: Thrity sparague-dawley (SD) rats were randomly divided into health group (A), osteoarthritis (OA) group (B) and experimental group (C). The rats of B group and C group (total 20 rats) were given intra-articular injection with the 20 μL mixture of 4% papain solution and 0.03 mol/L L-cysteine at the first day, second day and third day respectively, to establish the model of KOA. At the same time, the rats of C group were given intra-articular injection of 0.06 mL SH and 0.06 mL danshen once a week. The knee joint activity scopes of the rats were measured and then the rats were sacrificed at the fourth week after the first injections. The knee cartilages were cut for general observation and pathologically examination and the results were performed Mankin’s scores. Results: The knee joint activity scope of C group was significant larger than that of B group (P<0.01). Furthermore, the cartilage damage mitigated in C group, and the Mankin’s scores of C group improved and had significant difference when comparing with that of B group (P<0.05). Conclusion: The combination of danshen injection with SH injection could improve the knee joint activity scope, protect the cartilage structure, reduce the degree of cartilage degeneration, and alleviate the destruction of the cartilage cells and the pathology of KOA, but they could not inhibit the process of KOA completely.
Key words: traditional Chinese medcine; danshen injection; sodium hyaluronate; knee osteroarthritis; rat; experimental research
发表期数: 2012年12月第23期
引用格式: 尹明,曲良烨,贺荔枝,等. 丹参注射液联合透明质酸钠关节腔内注射治疗大鼠膝骨关节炎的实验研究[J]. 中国科技论文在线精品论文,2012,5(23):2273-2279.
 
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