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黄芪对低氧环境下SDF-1诱导hBMSCs迁移的干预作用研究

发表时间:2012-12-15  浏览量:1043  下载量:363
全部作者: 林冬阳,杨博华,沈凌
作者单位: 北京中医药大学东直门医院周围血管科;中国中医科学院广安门医院骨科
摘 要: 目的:研究模拟人体内低氧环境,黄芪和血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)及间质细胞衍生因子1(stromal cell-derived factor 1, SDF-1)等重要细胞因子对人骨髓间充质干细胞(human bone marrow mesenchymal stem cells, hBMSCs)向病灶迁移及向血管内皮细胞(vascular endothelial cells,VECs)分化能力的影响。方法:应用Transwell系统,实验细胞随机分为7 组。将常氧3组置于普通CO2细胞培养箱(含21%O2)中培养,低氧4组置于三气培养箱(含5%O2)中培养。培养48 h后,随机选择3个连续显微镜视野计数迁移到上室膜下的细胞,取平均数。结果:1)在常氧培养3组中,SDF+黄芪+常氧组与其他2组比较,hBMSCs迁移能力显著升高(P<0.01,P<0.05);2)在低氧培养4组中,SDF+黄芪+低氧组与其他3组比较,hBMSCs迁移能力显著升高(P<0.01);与SDF+低氧组比较,低氧对照组hBMSCs迁移能力显著升高(P<0.01);低氧对照组与黄芪+低氧组组间差异无统计学意义(P>0.05);3)与SDF+黄芪+常氧组比较,SDF+黄芪+低氧组hBMSCs迁移能力显著升高(P<0.05);SDF+常氧组与SDF+低氧组、黄芪+常氧组与黄芪+低氧组组间差异无统计学意义(P>0.05)。结论:在常氧和低氧环境中,黄芪与SDF-1联合使用可促进BMSCs迁移;而在低氧环境中,单独应用黄芪没有显著的促进作用。
关 键 词: 中医外科学;骨髓间充质干细胞;黄芪;间质细胞衍生因子-1;迁移;血管内皮细胞生长因子
Title: Study on the intervention of Astragalus on the hBMSCs migration induced by SDF-1 in hypoxic environment
Author: LIN Dongyang, YANG Bohua, SHEN Ling
Organization: Department of Peripheral Vessel, Dongzhimen Hospital, Beijing University of Chinese Medicine; Department of Orthopedics, Guang’anmen Hospital, China Academy of Chinese Medical Sciences
Abstract: Objective: To study the effects of Astragalus, vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1) on the human bone marrow mesenchymal stem cells (hBMSCs) capabilities of migrating to the lesion sites and differentiating into vascular endothelial cells (VECs) by simulating hypoxic environment of human body. Methods: Using Transwell system, the experimental cells were randomly divided into 7 groups. The three normxic groups were placed in ordinary cell culture incubator (including 21% O2); The four hypoxic groups were placed in the special cell culture incubator (including 5% O2). After 48-h culture, the cells were counted by randomly selecting three consecutive microscope visual fields below the membrane,and the final cell number was the average of three visions. Results: 1) Among the three normoxic groups,the ability of migration of hBMSCs in SDF+Astragalus+normoxic group was significantly higher than that of others (P<0.01,P<0.05); 2) Among the four hypoxic groups,the ability of migration of hBMSCs in SDF+Astragalus+hypoxic group was significantly higher than that of others (P<0.01);the ability of migration of hBMSCs in SDF+hypoxic group was significantly higher than that in hypoxic control group (P<0.01);the ability of migration of hBMSCs in hypoxic+Astragalus group and hypoxic control group had no significant difference (P>0.05); 3) The ability of migration of hBMSCs in SDF+Astragalus+hypoxic group was significantly higher than that in SDF+Astragalus+normoxic group (P<0.05); The ability of migration of hBMSCs in SDF+normoxic group and SDF+hypoxic group had no significant difference (P>0.05);The ability of migration of hBMSCs in Astragalus+normoxic group and Astragalus +hypoxic group also had no significant difference (P>0.05). Conclusion: In normoxic and hypoxic environments, Astragalus combined with SDF-1 could promote the migration of BMSCs. However, Astragalus alone could not promote the migration of BMSCs in the hypoxic environment.
Key words: surgery of traditional Chinese medicine; bone marrow mesenchymal stem cells; Astragalus; stromal cell-derived factor 1; migration; vascular endothelial growth factor
发表期数: 2012年12月第23期
引用格式: 林冬阳,杨博华,沈凌. 黄芪对低氧环境下SDF-1诱导hBMSCs迁移的干预作用研究[J]. 中国科技论文在线精品论文,2012,5(23):2285-2289.
 
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