您的位置：首页 > 论文页面
黄芪对低氧环境下SDF-1诱导hBMSCs迁移的干预作用研究
发表时间：2012-12-15 浏览量：1420 下载量：430
| 全部作者： | 林冬阳，杨博华，沈凌 |
| 作者单位： | 北京中医药大学东直门医院周围血管科；中国中医科学院广安门医院骨科 |
| 摘 要： | 目的：研究模拟人体内低氧环境，黄芪和血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）及间质细胞衍生因子1（stromal cell-derived factor 1， SDF-1）等重要细胞因子对人骨髓间充质干细胞（human bone marrow mesenchymal stem cells， hBMSCs）向病灶迁移及向血管内皮细胞（vascular endothelial cells，VECs）分化能力的影响。方法：应用Transwell系统，实验细胞随机分为7 组。将常氧3组置于普通CO2细胞培养箱（含21%O2）中培养，低氧4组置于三气培养箱（含5%O2）中培养。培养48 h后，随机选择3个连续显微镜视野计数迁移到上室膜下的细胞，取平均数。结果：1）在常氧培养3组中，SDF+黄芪+常氧组与其他2组比较，hBMSCs迁移能力显著升高（P<0.01，P<0.05）；2）在低氧培养4组中，SDF+黄芪+低氧组与其他3组比较，hBMSCs迁移能力显著升高（P<0.01）；与SDF+低氧组比较，低氧对照组hBMSCs迁移能力显著升高（P<0.01）；低氧对照组与黄芪+低氧组组间差异无统计学意义（P>0.05）；3）与SDF+黄芪+常氧组比较，SDF+黄芪+低氧组hBMSCs迁移能力显著升高（P<0.05）；SDF+常氧组与SDF+低氧组、黄芪+常氧组与黄芪+低氧组组间差异无统计学意义（P>0.05）。结论：在常氧和低氧环境中，黄芪与SDF-1联合使用可促进BMSCs迁移；而在低氧环境中，单独应用黄芪没有显著的促进作用。 |
| 关 键 词： | 中医外科学；骨髓间充质干细胞；黄芪；间质细胞衍生因子-1；迁移；血管内皮细胞生长因子 |
| Title： | Study on the intervention of Astragalus on the hBMSCs migration induced by SDF-1 in hypoxic environment |
| Author： | LIN Dongyang, YANG Bohua, SHEN Ling |
| Organization： | Department of Peripheral Vessel, Dongzhimen Hospital, Beijing University of Chinese Medicine; Department of Orthopedics, Guang’anmen Hospital, China Academy of Chinese Medical Sciences |
| Abstract： | Objective: To study the effects of Astragalus, vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1) on the human bone marrow mesenchymal stem cells (hBMSCs) capabilities of migrating to the lesion sites and differentiating into vascular endothelial cells (VECs) by simulating hypoxic environment of human body. Methods: Using Transwell system, the experimental cells were randomly divided into 7 groups. The three normxic groups were placed in ordinary cell culture incubator (including 21% O2); The four hypoxic groups were placed in the special cell culture incubator (including 5% O2). After 48-h culture, the cells were counted by randomly selecting three consecutive microscope visual fields below the membrane，and the final cell number was the average of three visions. Results: 1) Among the three normoxic groups，the ability of migration of hBMSCs in SDF+Astragalus+normoxic group was significantly higher than that of others (P<0.01，P<0.05); 2) Among the four hypoxic groups，the ability of migration of hBMSCs in SDF+Astragalus+hypoxic group was significantly higher than that of others (P<0.01)；the ability of migration of hBMSCs in SDF+hypoxic group was significantly higher than that in hypoxic control group (P<0.01)；the ability of migration of hBMSCs in hypoxic+Astragalus group and hypoxic control group had no significant difference (P>0.05); 3) The ability of migration of hBMSCs in SDF+Astragalus+hypoxic group was significantly higher than that in SDF+Astragalus+normoxic group (P<0.05); The ability of migration of hBMSCs in SDF+normoxic group and SDF+hypoxic group had no significant difference (P>0.05)；The ability of migration of hBMSCs in Astragalus+normoxic group and Astragalus +hypoxic group also had no significant difference (P>0.05). Conclusion: In normoxic and hypoxic environments, Astragalus combined with SDF-1 could promote the migration of BMSCs. However, Astragalus alone could not promote the migration of BMSCs in the hypoxic environment. |
| Key words： | surgery of traditional Chinese medicine; bone marrow mesenchymal stem cells; Astragalus; stromal cell-derived factor 1; migration; vascular endothelial growth factor |
| 发表期数： | 2012年12月第23期 |
| 引用格式： | 林冬阳，杨博华，沈凌. 黄芪对低氧环境下SDF-1诱导hBMSCs迁移的干预作用研究[J]. 中国科技论文在线精品论文，2012，5（23）：2285-2289. |
0
评论数 0请您登录
暂无评论
下载全文
多维论文